1iyi

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[[Image:1iyi.jpg|left|200px]]
[[Image:1iyi.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1iyi |SIZE=350|CAPTION= <scene name='initialview01'>1iyi</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1iyi", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1iyi| PDB=1iyi | SCENE= }}
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|RELATEDENTRY=[[1iyh|1IYH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iyi OCA], [http://www.ebi.ac.uk/pdbsum/1iyi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iyi RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE'''
'''CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE'''
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Inoue, T.]]
[[Category: Inoue, T.]]
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[[Category: gst]]
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[[Category: Gst]]
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[[Category: hematopoietic prostaglandin d synthase]]
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[[Category: Hematopoietic prostaglandin d synthase]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: pgd]]
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[[Category: Pgd]]
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[[Category: sigma-class gst]]
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[[Category: Sigma-class gst]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:35:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:26:27 2008''
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Revision as of 17:35, 2 May 2008

Image:1iyi.jpg

Template:STRUCTURE 1iyi

CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE


Overview

Here we report the crystal structures of human hematopoietic prostaglandin (PG) D synthase bound to glutathione (GSH) and Ca2+ or Mg2+. Using GSH as a cofactor, prostaglandin D synthase catalyzes the isomerization of PGH2 to PGD2, a mediator for allergy response. The enzyme is a homodimer, and Ca2+ or Mg2+ increases its activity to approximately 150% of the basal level, with half maximum effective concentrations of 400 microM for Ca2+ and 50 microM for Mg2+. In the Mg2+-bound form, the ion is octahedrally coordinated by six water molecules at the dimer interface. The water molecules are surrounded by pairs of Asp93, Asp96 and Asp97 from each subunit. Ca(2+) is coordinated by five water molecules and an Asp96 from one subunit. The Asp96 residue in the Ca2+-bound form makes hydrogen bonds with two guanidium nitrogen atoms of Arg14 in the GSH-binding pocket. Mg2+ alters the coordinating water structure and reduces one hydrogen bond between Asp96 and Arg14, thereby changing the interaction between Arg14 and GSH. This effect explains a four-fold reduction in the K(m) of the enzyme for GSH. The structure provides insights into how Ca2+ or Mg2+ binding activates human hematopoietic PGD synthase.

About this Structure

1IYI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Mechanism of metal activation of human hematopoietic prostaglandin D synthase., Inoue T, Irikura D, Okazaki N, Kinugasa S, Matsumura H, Uodome N, Yamamoto M, Kumasaka T, Miyano M, Kai Y, Urade Y, Nat Struct Biol. 2003 Apr;10(4):291-6. PMID:12627223 Page seeded by OCA on Fri May 2 20:35:04 2008

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