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| | ==Crystal structure of BglB with natural substrate== | | ==Crystal structure of BglB with natural substrate== |
| - | <StructureSection load='4hz8' size='340' side='right' caption='[[4hz8]], [[Resolution|resolution]] 1.14Å' scene=''> | + | <StructureSection load='4hz8' size='340' side='right'caption='[[4hz8]], [[Resolution|resolution]] 1.14Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hz8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacterial_sp. Bacterial sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3fj0 3fj0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZ8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hz8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3fj0 3fj0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZ8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hz6|4hz6]], [[4hz7|4hz7]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz8 OCA], [https://pdbe.org/4hz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hz8 RCSB], [https://www.ebi.ac.uk/pdbsum/4hz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hz8 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bglA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77133 Bacterial sp.])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz8 OCA], [http://pdbe.org/4hz8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hz8 RCSB], [http://www.ebi.ac.uk/pdbsum/4hz8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hz8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0GMU3_9BACT Q0GMU3_9BACT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Beta-glucosidase|Beta-glucosidase]] | + | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterial sp]] | + | [[Category: Large Structures]] |
| - | [[Category: Hwang, K Y]] | + | [[Category: Uncultured bacterium]] |
| - | [[Category: Nam, K H]] | + | [[Category: Hwang KY]] |
| - | [[Category: Beta-glucosidase]] | + | [[Category: Nam KH]] |
| - | [[Category: Bgl]]
| + | |
| - | [[Category: Bglb]]
| + | |
| - | [[Category: Carbohydrate/sugar binding]]
| + | |
| - | [[Category: Glucosidase]]
| + | |
| - | [[Category: Glycosidase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Tim barrel]]
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| Structural highlights
Function
Q0GMU3_9BACT
Publication Abstract from PubMed
Beta-glucosidase enzymes (EC 3.2.1-3.2.3) hydrolyze sugars and are implicated in a wide spectrum of biological processes. Recently, we reported that beta-glucosidase has varied kinetic parameters for the natural and synthetic substrates [K.H Nam, S.J. Kim, M.Y. Kim, J.H. Kim, T.S. Yeo, C.M. Lee, H.K Jun, K.Y. Hwang. Crystal structure of engineered beta-glucosidase from a soil metagenome, Proteins 73 (2008) 788-793]. However, an understanding of the kinetic values of beta-glucosidase has not yet enabled the elucidation of its molecular function. Here, we report the X-ray crystal structure of beta-glucosidase with a glucose and cellobiose fragment from uncultured soil metagenome. From the various crystals, we obtained the pre-reaction (native), intermediate (disaccharide cleavage) and post-reaction (glucose binding) states of the active site pocket. These structures provide snapshots of the catalytic processing of beta-glucosidase. In addition, the intermediate state of the crystal structure provides insight into the substrate specificity of beta-glucosidase. These structural studies will facilitate elucidation of the architectural mechanism responsible for the substrate recognition of beta-glucosidase.
Structural insights into the substrate recognition properties of beta-glucosidase.,Nam KH, Sung MW, Hwang KY Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5. doi:, 10.1016/j.bbrc.2009.12.038. Epub 2009 Dec 11. PMID:20005197[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nam KH, Sung MW, Hwang KY. Structural insights into the substrate recognition properties of beta-glucosidase. Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5. doi:, 10.1016/j.bbrc.2009.12.038. Epub 2009 Dec 11. PMID:20005197 doi:http://dx.doi.org/10.1016/j.bbrc.2009.12.038
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