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| | ==Insulin protein crystallization via langmuir-blodgett== | | ==Insulin protein crystallization via langmuir-blodgett== |
| - | <StructureSection load='4i5z' size='340' side='right' caption='[[4i5z]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='4i5z' size='340' side='right'caption='[[4i5z]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4i5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I5Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4i5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I5Z FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bn1|2bn1]], [[4i5y|4i5y]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i5z OCA], [https://pdbe.org/4i5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i5z RCSB], [https://www.ebi.ac.uk/pdbsum/4i5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i5z ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">INS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i5z OCA], [http://pdbe.org/4i5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i5z RCSB], [http://www.ebi.ac.uk/pdbsum/4i5z PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. | + | [https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Molecular Playground/Insulin|Molecular Playground/Insulin]] | + | *[[Insulin 3D Structures|Insulin 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bovin]] | + | [[Category: Bos taurus]] |
| - | [[Category: Belmonte, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Bragazzi, N]] | + | [[Category: Belmonte L]] |
| - | [[Category: Nicolini, C]] | + | [[Category: Bragazzi N]] |
| - | [[Category: Pechkova, E]] | + | [[Category: Nicolini C]] |
| - | [[Category: Hormone]]
| + | [[Category: Pechkova E]] |
| - | [[Category: Langmuir blodgett]]
| + | |
| - | [[Category: Optimal crystallization]]
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| - | [[Category: Space grown]]
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| - | [[Category: Thin film]]
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| Structural highlights
Function
INS_BOVIN Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Publication Abstract from PubMed
Crystallization is a highly demanding and time-consuming task that causes a real bottle-neck in basic research. Great effort has been made to understand the factors and parameters that influence this process and to finely tune them to facilitate crystal growth. Different crystallization techniques have been proposed over the past decades, such as the classical vapor hanging drop method, its variant the sitting drop method, dialysis, cryo-temperature, gel, batch, and the innovative microgravity (space) techniques like free interface diffusion (FID) and counter-ion diffusion (CID). Here, we present a review of the strategies utilizing Langmuir-Blodgett (LB)-based nanotechnologies, and microgravity techniques for obtaining optimal high-quality crystals, as proven by molecular dynamics (MD) and bioinformatics approaches, namely using a clustering algorithm and protein alignment.
A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity.,Pechkova E, Bragazzi N, Bozdaganyan M, Belmonte L, Nicolini C Crit Rev Eukaryot Gene Expr. 2014;24(4):325-39. PMID:25403962[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pechkova E, Bragazzi N, Bozdaganyan M, Belmonte L, Nicolini C. A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity. Crit Rev Eukaryot Gene Expr. 2014;24(4):325-39. PMID:25403962
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