1izj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1izj.jpg|left|200px]]
[[Image:1izj.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1izj |SIZE=350|CAPTION= <scene name='initialview01'>1izj</scene>, resolution 2.20&Aring;
+
The line below this paragraph, containing "STRUCTURE_1izj", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1izj| PDB=1izj | SCENE= }}
-
|RELATEDENTRY=[[1ji1|1JI1]], [[1izk|1IZK]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1izj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1izj OCA], [http://www.ebi.ac.uk/pdbsum/1izj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1izj RCSB]</span>
+
-
}}
+
'''Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a'''
'''Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a'''
Line 32: Line 29:
[[Category: Sakano, Y.]]
[[Category: Sakano, Y.]]
[[Category: Tonozuka, T.]]
[[Category: Tonozuka, T.]]
-
[[Category: alpha-beta barrele]]
+
[[Category: Alpha-beta barrele]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:37:17 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:26:55 2008''
+

Revision as of 17:37, 2 May 2008

Image:1izj.jpg

Template:STRUCTURE 1izj

Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a


Overview

Thermoactinomyces vulgaris R-47 produces two alpha-amylases, TVAI and TVAII, differing in substrate specificity from each other. TVAI favors high-molecular-weight substrates like starch, and scarcely hydrolyzes cyclomaltooligosaccharides (cyclodextrins) with a small cavity. TVAII favors low-molecular-weight substrates like oligosaccharides, and can efficiently hydrolyze cyclodextrins with various sized cavities. To understand the relationship between the structure and substrate specificity of these enzymes, we precisely examined the roles of key residues for substrate recognition by X-ray structural and kinetic parameter analyses of mutant enzymes and successfully obtained mutants in which the substrate specificity of each enzyme is partially converted into that of another.

About this Structure

1IZJ is a Single protein structure of sequence from Thermoactinomyces vulgaris. Full crystallographic information is available from OCA.

Reference

Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 alpha-amylases TVAI and TVAII by site-directed mutagenesis., Ohtaki A, Iguchi A, Mizuno M, Tonozuka T, Sakano Y, Kamitori S, Carbohydr Res. 2003 Jul 22;338(15):1553-8. PMID:12860426 Page seeded by OCA on Fri May 2 20:37:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1izj"
Personal tools