7ya2
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of capsular polysaccharide synthesis enzyme CapG from Staphylococcus aureus== | |
| + | <StructureSection load='7ya2' size='340' side='right'caption='[[7ya2]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7ya2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YA2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ya2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ya2 OCA], [https://pdbe.org/7ya2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ya2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ya2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ya2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3K5M7_STAAE A0A0H3K5M7_STAAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial capsular polysaccharides provide protection against environmental stress and immune evasion from the host immune system, and are therefore considered to be attractive therapeutic targets for the development of anti-infectious reagents. Here, we focused on CapG, one of the key enzymes in the synthesis pathway of capsular polysaccharides type 5 (CP5) from the opportunistic pathogen Staphylococcus aureus. SaCapG catalyses the 2-epimerization of UDP-N-acetyl-D-talosamine (UDP-TalNAc) to UDP-N-acetyl-D-fucosamine (UDP-FucNAc), which is one of the nucleotide-activated precursors for the synthesis of the trisaccharide repeating units of CP5. Here, the cloning, expression and purification of recombinant SaCapG are reported. After extensive efforts, single crystals of SaCapG were successfully obtained which belonged to space group C2 and exhibited unit-cell parameters a = 302.91, b = 84.34, c = 145.09 A, beta = 110.65 degrees . The structure was solved by molecular replacement and was refined to 3.2 A resolution. The asymmetric unit revealed a homohexameric assembly of SaCapG, which was consistent with gel-filtration analysis. Structural comparison with UDP-N-acetyl-D-glucosamine 2-epimerase from Methanocaldococcus jannaschii identified alpha2, the alpha2-alpha3 loop and alpha10 as a gate-regulated switch controlling substrate entry and/or product release. | ||
| - | + | Crystal structure of the capsular polysaccharide-synthesis enzyme CapG from Staphylococcus aureus.,Tien N, Ho CY, Lai SJ, Lin YC, Yang CS, Wang YC, Huang WC, Chen Y, Chang JJ Acta Crystallogr F Struct Biol Commun. 2022 Nov 1;78(Pt 11):378-385. doi:, 10.1107/S2053230X22008743. Epub 2022 Oct 14. PMID:36322423<ref>PMID:36322423</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7ya2" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus str. Newman]] | ||
| + | [[Category: Chen Y]] | ||
| + | [[Category: Wang YC]] | ||
Revision as of 20:08, 16 November 2022
Crystal structure of capsular polysaccharide synthesis enzyme CapG from Staphylococcus aureus
| |||||||||||
