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| | ==The Phenylacetyl-CoA monooxygenase - mutant PaaA E49Q K68Q - PaaC wild type subcomplex with benzoyl-CoA== | | ==The Phenylacetyl-CoA monooxygenase - mutant PaaA E49Q K68Q - PaaC wild type subcomplex with benzoyl-CoA== |
| - | <StructureSection load='4ii4' size='340' side='right' caption='[[4ii4]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='4ii4' size='340' side='right'caption='[[4ii4]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ii4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_mg1655 Escherichia coli mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4II4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4II4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ii4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4II4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4II4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BYC:BENZOYL+COENZYME+A'>BYC</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BYC:BENZOYL+COENZYME+A'>BYC</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pvr|3pvr]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ii4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ii4 OCA], [https://pdbe.org/4ii4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ii4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ii4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ii4 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1388, JW1383, paaA, ydbO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655]), b1390, JW1385, paaC, ydbP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylacetyl-CoA_1,2-epoxidase Phenylacetyl-CoA 1,2-epoxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.149 1.14.13.149] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ii4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ii4 OCA], [http://pdbe.org/4ii4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ii4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ii4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ii4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PAAA_ECOLI PAAA_ECOLI]] Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.<ref>PMID:9748275</ref> <ref>PMID:16997993</ref> <ref>PMID:20660314</ref> <ref>PMID:21247899</ref> [[http://www.uniprot.org/uniprot/PAAC_ECOLI PAAC_ECOLI]] Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit C may be essential for structural integrity of the alpha subunit.<ref>PMID:9748275</ref> <ref>PMID:16997993</ref> <ref>PMID:20660314</ref> | + | [https://www.uniprot.org/uniprot/PAAA_ECOLI PAAA_ECOLI] Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.<ref>PMID:9748275</ref> <ref>PMID:16997993</ref> <ref>PMID:20660314</ref> <ref>PMID:21247899</ref> |
| | + | |
| | + | ==See Also== |
| | + | *[[Epoxidase 3D structures|Epoxidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Escherichia coli mg1655]] | + | [[Category: Escherichia coli str. K-12 substr. MG1655]] |
| - | [[Category: Phenylacetyl-CoA 1,2-epoxidase]]
| + | [[Category: Large Structures]] |
| - | [[Category: Structural genomic]] | + | [[Category: Cygler M]] |
| - | [[Category: Cygler, M]] | + | [[Category: Grishin AM]] |
| - | [[Category: Grishin, A M]] | + | |
| - | [[Category: Bacterial multicomponent monooxygenase paaabce]]
| + | |
| - | [[Category: Bsgi]]
| + | |
| - | [[Category: Ferritin-like fold]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Protein-protein complex]]
| + | |
| Structural highlights
Function
PAAA_ECOLI Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.[1] [2] [3] [4]
See Also
References
- ↑ Ferrandez A, Minambres B, Garcia B, Olivera ER, Luengo JM, Garcia JL, Diaz E. Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway. J Biol Chem. 1998 Oct 2;273(40):25974-86. PMID:9748275
- ↑ Fernandez C, Ferrandez A, Minambres B, Diaz E, Garcia JL. Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli. Appl Environ Microbiol. 2006 Nov;72(11):7422-6. Epub 2006 Sep 22. PMID:16997993 doi:10.1128/AEM.01550-06
- ↑ Teufel R, Mascaraque V, Ismail W, Voss M, Perera J, Eisenreich W, Haehnel W, Fuchs G. Bacterial phenylalanine and phenylacetate catabolic pathway revealed. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14390-5. doi:, 10.1073/pnas.1005399107. Epub 2010 Jul 21. PMID:20660314 doi:10.1073/pnas.1005399107
- ↑ Grishin AM, Ajamian E, Tao L, Zhang L, Menard R, Cygler M. Structural and functional studies of the Escherichia coli phenylacetyl-coa monooxygenase complex. J Biol Chem. 2011 Jan 19. PMID:21247899 doi:10.1074/jbc.M110.194423
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