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| | ==Crystal structure of an Aar2p S253E phosphomimetic mutant protein== | | ==Crystal structure of an Aar2p S253E phosphomimetic mutant protein== |
| - | <StructureSection load='4ili' size='340' side='right' caption='[[4ili]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='4ili' size='340' side='right'caption='[[4ili]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ili]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ILI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ili]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ilg|4ilg]], [[4ilh|4ilh]], [[4ilj|4ilj]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ili FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ili OCA], [https://pdbe.org/4ili PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ili RCSB], [https://www.ebi.ac.uk/pdbsum/4ili PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ili ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AAR2, YBL074C, YBL06.06, YBL0611 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ili FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ili OCA], [http://pdbe.org/4ili PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ili RCSB], [http://www.ebi.ac.uk/pdbsum/4ili PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ili ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AAR2_YEAST AAR2_YEAST]] Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth. | + | [https://www.uniprot.org/uniprot/AAR2_YEAST AAR2_YEAST] Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Heroven, C]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Santos, K F]] | + | [[Category: Heroven C]] |
| - | [[Category: Wahl, M C]]
| + | [[Category: Santos KF]] |
| - | [[Category: Weber, G]] | + | [[Category: Wahl MC]] |
| - | [[Category: Aar2]] | + | [[Category: Weber G]] |
| - | [[Category: Prp8]] | + | |
| - | [[Category: Splicing]]
| + | |
| - | [[Category: U5 snrnp assembly]]
| + | |
| Structural highlights
Function
AAR2_YEAST Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth.
Publication Abstract from PubMed
Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase. Instead, pre-U5 snRNP includes the Aar2 protein not found in mature U5 snRNP or spliceosomes. Aar2p and Brr2p bind competitively to a C-terminal region of Prp8p that comprises consecutive RNase H-like and Jab1/MPN-like domains. To elucidate the molecular basis for this competition, we determined the crystal structure of Aar2p in complex with the Prp8p RNase H and Jab1/MPN domains. Aar2p binds on one side of the RNase H domain and extends its C terminus to the other side, where the Jab1/MPN domain is docked onto a composite Aar2p-RNase H platform. Known Brr2p interaction sites of the Jab1/MPN domain remain available, suggesting that Aar2p-mediated compaction of the Prp8p domains sterically interferes with Brr2p binding. Moreover, Aar2p occupies known RNA-binding sites of the RNase H domain, and Aar2p interferes with binding of U4/U6 di-snRNA to the Prp8p C-terminal region. Structural and functional analyses of phospho-mimetic mutations reveal how phosphorylation reduces affinity of Aar2p for Prp8p and allows Brr2p and U4/U6 binding. Our results show how Aar2p regulates both protein and RNA binding to Prp8p during U5 snRNP assembly.
Structural basis for dual roles of Aar2p in U5 snRNP assembly.,Weber G, Cristao VF, Santos KF, Jovin SM, Heroven AC, Holton N, Luhrmann R, Beggs JD, Wahl MC Genes Dev. 2013 Mar 1;27(5):525-40. doi: 10.1101/gad.213207.113. Epub 2013 Feb, 26. PMID:23442228[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Weber G, Cristao VF, Santos KF, Jovin SM, Heroven AC, Holton N, Luhrmann R, Beggs JD, Wahl MC. Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes Dev. 2013 Mar 1;27(5):525-40. doi: 10.1101/gad.213207.113. Epub 2013 Feb, 26. PMID:23442228 doi:10.1101/gad.213207.113
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