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| | ==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6== | | ==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6== |
| - | <StructureSection load='4ill' size='340' side='right' caption='[[4ill]], [[Resolution|resolution]] 2.48Å' scene=''> | + | <StructureSection load='4ill' size='340' side='right'caption='[[4ill]], [[Resolution|resolution]] 2.48Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ill]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ILL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ill]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILL FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ilm|4ilm]], [[4ilr|4ilr]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ill FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ill OCA], [https://pdbe.org/4ill PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ill RCSB], [https://www.ebi.ac.uk/pdbsum/4ill PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ill ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ill FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ill OCA], [http://pdbe.org/4ill PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ill RCSB], [http://www.ebi.ac.uk/pdbsum/4ill PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ill ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CAS6B_SACS2 CAS6B_SACS2] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease, generating an 8 base-long tag known as the 5' handle.<ref>PMID:21507944</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Endonuclease|Endonuclease]] | + | *[[CRISPR subtype I-A|CRISPR subtype I-A]] |
| | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sulso]] | + | [[Category: Large Structures]] |
| - | [[Category: Li, H]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Shao, Y]] | + | [[Category: Li H]] |
| - | [[Category: 2'-deoxy modification]] | + | [[Category: Shao Y]] |
| - | [[Category: Cas6]]
| + | |
| - | [[Category: Hydrolase-rna complex]]
| + | |
| - | [[Category: Rna]]
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| - | [[Category: Rna cleavage]]
| + | |
| - | [[Category: U16]]
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| Structural highlights
Function
CAS6B_SACS2 CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease, generating an 8 base-long tag known as the 5' handle.[1]
Publication Abstract from PubMed
Clustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing.
Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6.,Shao Y, Li H Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lintner NG, Kerou M, Brumfield SK, Graham S, Liu H, Naismith JH, Sdano M, Peng N, She Q, Copie V, Young MJ, White MF, Lawrence CM. Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE). J Biol Chem. 2011 Jun 17;286(24):21643-56. Epub 2011 Apr 20. PMID:21507944 doi:10.1074/jbc.M111.238485
- ↑ Shao Y, Li H. Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6. Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186 doi:http://dx.doi.org/10.1016/j.str.2013.01.010
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