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| | ==Crystal structure of amino acid residues 1-120 of CG17282== | | ==Crystal structure of amino acid residues 1-120 of CG17282== |
| - | <StructureSection load='4it6' size='340' side='right' caption='[[4it6]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4it6' size='340' side='right'caption='[[4it6]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4it6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IT6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IT6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4it6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IT6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4it6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4it6 OCA], [https://pdbe.org/4it6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4it6 RCSB], [https://www.ebi.ac.uk/pdbsum/4it6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4it6 ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4it4|4it4]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG17282, Dmel_CG17282 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4it6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4it6 OCA], [http://pdbe.org/4it6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4it6 RCSB], [http://www.ebi.ac.uk/pdbsum/4it6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4it6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9VDE4_DROME Q9VDE4_DROME] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Agyekum, B]] | + | [[Category: Large Structures]] |
| - | [[Category: Bouyain, S]] | + | [[Category: Agyekum B]] |
| - | [[Category: Hall, D R]] | + | [[Category: Bouyain S]] |
| - | [[Category: Fk506-binding protein]] | + | [[Category: Hall DR]] |
| - | [[Category: Ubiquitous]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
Q9VDE4_DROME
Publication Abstract from PubMed
The kinase DOUBLETIME is a master regulator of the Drosophila circadian clock, yet the mechanisms regulating its activity remain unclear. A proteomic analysis of DOUBLETIME interactors led to the identification of an unstudied protein designated CG17282. RNAi-mediated knockdown of CG17282 produced behavioral arrhythmicity and long periods and high levels of hypophosphorylated nuclear PERIOD and phosphorylated DOUBLETIME. Overexpression of DOUBLETIME in flies suppresses these phenotypes and overexpression of CG17282 in S2 cells enhances DOUBLETIME-dependent PERIOD degradation, indicating that CG17282 stimulates DOUBLETIME's circadian function. In photoreceptors, CG17282 accumulates rhythmically in PERIOD- and DOUBLETIME-dependent cytosolic foci. Finally, structural analyses demonstrated CG17282 is a noncanonical FK506-binding protein with an inactive peptide prolyl-isomerase domain that binds DOUBLETIME and tetratricopeptide repeats that may promote assembly of larger protein complexes. We have named CG17282 BRIDE OF DOUBLETIME and established it as a mediator of DOUBLETIME's effects on PERIOD, most likely in cytosolic foci that regulate PERIOD nuclear accumulation.
Noncanonical FK506-Binding Protein BDBT Binds DBT to Enhance Its Circadian Function and Forms Foci at Night.,Fan JY, Agyekum B, Venkatesan A, Hall DR, Keightley A, Bjes ES, Bouyain S, Price JL Neuron. 2013 Nov 20;80(4):984-96. doi: 10.1016/j.neuron.2013.08.004. Epub 2013, Nov 7. PMID:24210908[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fan JY, Agyekum B, Venkatesan A, Hall DR, Keightley A, Bjes ES, Bouyain S, Price JL. Noncanonical FK506-Binding Protein BDBT Binds DBT to Enhance Its Circadian Function and Forms Foci at Night. Neuron. 2013 Nov 20;80(4):984-96. doi: 10.1016/j.neuron.2013.08.004. Epub 2013, Nov 7. PMID:24210908 doi:http://dx.doi.org/10.1016/j.neuron.2013.08.004
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