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| | ==Structure of Rrp9== | | ==Structure of Rrp9== |
| - | <StructureSection load='4j0x' size='340' side='right' caption='[[4j0x]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4j0x' size='340' side='right'caption='[[4j0x]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j0x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J0X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j0x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J0X FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RRP9, YPR137W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0x OCA], [https://pdbe.org/4j0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j0x RCSB], [https://www.ebi.ac.uk/pdbsum/4j0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0x ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0x OCA], [http://pdbe.org/4j0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j0x RCSB], [http://www.ebi.ac.uk/pdbsum/4j0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0x ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RRP9_YEAST RRP9_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for efficient pre-rRNA cleavage at sites A0, A1 and A2, and biosynthesis of 18S rRNA.<ref>PMID:11105764</ref> | + | [https://www.uniprot.org/uniprot/RRP9_YEAST RRP9_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for efficient pre-rRNA cleavage at sites A0, A1 and A2, and biosynthesis of 18S rRNA.<ref>PMID:11105764</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Lin, J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ye, K]] | + | [[Category: Lin J]] |
| - | [[Category: Zhang, L]] | + | [[Category: Ye K]] |
| - | [[Category: Beta-propeller]] | + | [[Category: Zhang L]] |
| - | [[Category: Preribosome]]
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| - | [[Category: Rna binding protein]]
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| - | [[Category: Wd domain]]
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| Structural highlights
Function
RRP9_YEAST Involved in nucleolar processing of pre-18S ribosomal RNA. Required for efficient pre-rRNA cleavage at sites A0, A1 and A2, and biosynthesis of 18S rRNA.[1]
Publication Abstract from PubMed
The U3 snoRNA is required for 18S rRNA processing and small subunit ribosome formation in eukaryotes. Different from other box C/D snoRNAs, U3 contains an extra 5' domain that pairs with pre-rRNA and a unique B/C motif essential for recruitment of the U3-specific Rrp9 protein. Here, we analyze the structure and function of Rrp9 with crystallographic, biochemical, and cellular approaches. Rrp9 is composed of a WD repeat domain and an N-terminal region. The crystal structures of the WD domain of yeast Rrp9 and its human ortholog U3-55K were determined, revealing a typical seven-bladed propeller fold. Several conserved surface patches on the WD domain were identified, and their function in RNP assembly and yeast growth were analyzed by mutagenesis. Prior association of Snu13 with the B/C motif was found to enhance the specific binding of the WD domain. We show that a conserved 7bc loop is crucial for specific recognition of U3, nucleolar localization of Rrp9, and yeast growth. In addition, we show that the N-terminal region of Rrp9 contains a bipartite nuclear localization signal that is dispensable for nucleolar localization. Our results provide insight into the functional sites of Rrp9.
Structural and functional analysis of the U3 snoRNA binding protein Rrp9.,Zhang L, Lin J, Ye K RNA. 2013 May;19(5):701-11. doi: 10.1261/rna.037580.112. Epub 2013 Mar 18. PMID:23509373[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Venema J, Vos HR, Faber AW, van Venrooij WJ, Raue HA. Yeast Rrp9p is an evolutionarily conserved U3 snoRNP protein essential for early pre-rRNA processing cleavages and requires box C for its association. RNA. 2000 Nov;6(11):1660-71. PMID:11105764
- ↑ Zhang L, Lin J, Ye K. Structural and functional analysis of the U3 snoRNA binding protein Rrp9. RNA. 2013 May;19(5):701-11. doi: 10.1261/rna.037580.112. Epub 2013 Mar 18. PMID:23509373 doi:10.1261/rna.037580.112
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