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| | ==Atg13 HORMA domain== | | ==Atg13 HORMA domain== |
| - | <StructureSection load='4j2g' size='340' side='right' caption='[[4j2g]], [[Resolution|resolution]] 2.29Å' scene=''> | + | <StructureSection load='4j2g' size='340' side='right'caption='[[4j2g]], [[Resolution|resolution]] 2.29Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_thermotolerans Kluyveromyces thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachancea_thermotolerans_CBS_6340 Lachancea thermotolerans CBS 6340]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J2G FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2g OCA], [https://pdbe.org/4j2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j2g RCSB], [https://www.ebi.ac.uk/pdbsum/4j2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2g ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLTH0A00704g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2g OCA], [http://pdbe.org/4j2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j2g RCSB], [http://www.ebi.ac.uk/pdbsum/4j2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2g ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C5DB94_LACTC C5DB94_LACTC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Kluyveromyces thermotolerans]] | + | [[Category: Lachancea thermotolerans CBS 6340]] |
| - | [[Category: Hurley, J H]] | + | [[Category: Large Structures]] |
| - | [[Category: Jao, C]] | + | [[Category: Hurley JH]] |
| - | [[Category: Ragusa, M J]] | + | [[Category: Jao C]] |
| - | [[Category: Stanley, R E]] | + | [[Category: Ragusa MJ]] |
| - | [[Category: Atg13]] | + | [[Category: Stanley RE]] |
| - | [[Category: Autophagy]]
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| - | [[Category: Horma]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
C5DB94_LACTC
Publication Abstract from PubMed
Autophagy-related 13 (Atg13) is a key early-acting factor in autophagy and the major locus for nutrient-dependent regulation of autophagy by Tor. The 2.3-A resolution crystal structure of the N-terminal domain of Atg13 reveals a previously unidentified HORMA (Hop1p, Rev1p and Mad2) domain similar to that of the spindle checkpoint protein Mad2. Mad2 has two different stable conformations, O-Mad2 and C-Mad2, and the Atg13 HORMA structure corresponds to the C-Mad2 state. The Atg13 HORMA domain is required for autophagy and for recruitment of the phosphatidylinositol (PI) 3-kinase subunit Atg14 but is not required for Atg1 interaction or Atg13 recruitment to the preautophagosomal structure. The Atg13 HORMA structure reveals a pair of conserved Arg residues that constitute a putative phosphate sensor. One of the Arg residues is in the region corresponding to the "safety belt" conformational switch of Mad2, suggesting conformational regulation of phosphate binding. These two Arg residues are essential for autophagy, suggesting that the Atg13 HORMA domain could function as a phosphoregulated conformational switch.
A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy.,Jao CC, Ragusa MJ, Stanley RE, Hurley JH Proc Natl Acad Sci U S A. 2013 Mar 18. PMID:23509291[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jao CC, Ragusa MJ, Stanley RE, Hurley JH. A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy. Proc Natl Acad Sci U S A. 2013 Mar 18. PMID:23509291 doi:http://dx.doi.org/10.1073/pnas.1220306110
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