1hjb

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Revision as of 15:12, 12 November 2007

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spinqualitylabelsall models 1hjb, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

Overview

The core binding factor (CBF) heterodimeric transcription factors, comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta subunits are, essential for differentiation of hematopoietic and bone cells, and their, mutation is intimately related to the development of acute leukemias and, cleidocranial dysplasia. Here, we present the crystal structures of the, AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core, domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt, domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA, complexes. The hydrogen bonding network formed among CBFalpha(Runt domain), and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric, regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. The, point mutations of CBFalpha related to the aforementioned diseases were, also mapped and their effect on DNA binding is discussed.

About this Structure

1HJB is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta., Tahirov TH, Inoue-Bungo T, Morii H, Fujikawa A, Sasaki M, Kimura K, Shiina M, Sato K, Kumasaka T, Yamamoto M, Ishii S, Ogata K, Cell. 2001 Mar 9;104(5):755-67. PMID:11257229

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