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| | ==Crystal Structure of a parasite tRNA synthetase, ligand-free== | | ==Crystal Structure of a parasite tRNA synthetase, ligand-free== |
| - | <StructureSection load='4j76' size='340' side='right' caption='[[4j76]], [[Resolution|resolution]] 2.34Å' scene=''> | + | <StructureSection load='4j76' size='340' side='right'caption='[[4j76]], [[Resolution|resolution]] 2.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j76]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J76 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j76]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J76 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF13_0205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j76 OCA], [https://pdbe.org/4j76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j76 RCSB], [https://www.ebi.ac.uk/pdbsum/4j76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j76 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j76 OCA], [http://pdbe.org/4j76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j76 RCSB], [http://www.ebi.ac.uk/pdbsum/4j76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j76 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8IDW3_PLAF7 Q8IDW3_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4j76" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4j76" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plaf7]] | + | [[Category: Large Structures]] |
| - | [[Category: Tryptophan--tRNA ligase]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Hol, W G.J]] | + | [[Category: Hol WGJ]] |
| - | [[Category: Kim, J E]] | + | [[Category: Kim JE]] |
| - | [[Category: Koh, C Y]] | + | [[Category: Koh CY]] |
| - | [[Category: Verlinde, C L.M J]] | + | [[Category: Verlinde CLMJ]] |
| - | [[Category: Aar]]
| + | |
| - | [[Category: Aminoacyl-trna synthetase]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Nucleotide binding]]
| + | |
| - | [[Category: Parasite]]
| + | |
| - | [[Category: Protein-substrate complex]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Trpr]]
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| Structural highlights
Function
Q8IDW3_PLAF7
Publication Abstract from PubMed
Malaria, most commonly caused by the parasite Plasmodium falciparum, is a devastating disease that remains a large global health burden. Lack of vaccines and drug resistance necessitate the continual development of new drugs and exploration of new drug targets. Due to their essential role in protein synthesis, aminoacyl-tRNA synthetases are potential anti-malaria drug targets. Here we report the crystal structures of P. falciparum cytosolic tryptophanyl-tRNA synthetase (Pf-cTrpRS) in its ligand-free state and tryptophanyl-adenylate (WAMP)-bound state at 2.34A and 2.40A resolutions, respectively. Large conformational changes are observed when the ligand-free protein is bound to WAMP. Multiple residues, completely surrounding the active site pocket, collapse onto WAMP. Comparison of the structures to those of human cytosolic TrpRS (Hs-cTrpRS) provides information about the possibility of targeting Pf-cTrpRS for inhibitor development. There is a high degree of similarity between Pf-cTrpRS and Hs-cTrpRS within the active site. However, the large motion that Pf-cTrpRS undergoes during transitions between different functional states avails an opportunity to arrive at compounds which selectively perturb the motion, and may provide a starting point for the development of new anti-malaria therapeutics.
Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design.,Koh CY, Kim JE, Napoli AJ, Verlinde CL, Fan E, Buckner FS, Van Voorhis WC, Hol WG Mol Biochem Parasitol. 2013 May 7;189(1-2):26-32. doi:, 10.1016/j.molbiopara.2013.04.007. PMID:23665145[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Koh CY, Kim JE, Napoli AJ, Verlinde CL, Fan E, Buckner FS, Van Voorhis WC, Hol WG. Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design. Mol Biochem Parasitol. 2013 May 7;189(1-2):26-32. doi:, 10.1016/j.molbiopara.2013.04.007. PMID:23665145 doi:10.1016/j.molbiopara.2013.04.007
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