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| | ==Structure of the P. falciparum PFI1780w PHIST domain== | | ==Structure of the P. falciparum PFI1780w PHIST domain== |
| - | <StructureSection load='4jle' size='340' side='right' caption='[[4jle]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='4jle' size='340' side='right'caption='[[4jle]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4jle]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JLE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4jle]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JLE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFI1780w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jle OCA], [https://pdbe.org/4jle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jle RCSB], [https://www.ebi.ac.uk/pdbsum/4jle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jle ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jle OCA], [http://pdbe.org/4jle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jle RCSB], [http://www.ebi.ac.uk/pdbsum/4jle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jle ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8I2F2_PLAF7 Q8I2F2_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plaf7]] | + | [[Category: Large Structures]] |
| - | [[Category: Slater, L]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Vakonakis, I]]
| + | [[Category: Slater L]] |
| - | [[Category: 3-helix bundle]]
| + | [[Category: Vakonakis I]] |
| - | [[Category: Human p. falciparum-infected erythrocyte]]
| + | |
| - | [[Category: Pfemp1 ats domain]] | + | |
| - | [[Category: Protein binding]] | + | |
| - | [[Category: Protein interaction module]]
| + | |
| Structural highlights
Function
Q8I2F2_PLAF7
Publication Abstract from PubMed
Uniquely among malaria parasites, Plasmodium falciparum-infected erythrocytes (iRBCs) develop membrane protrusions, known as knobs, where the parasite adhesion receptor P. falciparum erythrocyte membrane protein 1 (PfEMP1) clusters. Knob formation and the associated iRBC adherence to host endothelium are directly linked to the severity of malaria and are functional manifestations of protein export from the parasite to the iRBC. A family of exported proteins featuring Plasmodium helical interspersed subtelomeric (PHIST) domains has attracted attention, with members being implicated in host-parasite protein interactions and differentially regulated in severe disease and among parasite isolates. Here, we show that PHIST member PFE1605w binds the PfEMP1 intracellular segment directly with Kd = 5 +/- 0.6 muM, comigrates with PfEMP1 during export, and locates in knobs. PHIST variants that do not locate in knobs (MAL8P1.4) or bind PfEMP1 30 times more weakly (PFI1780w) used as controls did not display the same pattern. We resolved the first crystallographic structure of a PHIST protein and derived a partial model of the PHIST-PfEMP1 interaction from nuclear magnetic resonance. We propose that PFE1605w reinforces the PfEMP1-cytoskeletal connection in knobs and discuss the possible role of PHIST proteins as interaction hubs in the parasite exportome.
A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 and comigrates to knobs on the host cell surface.,Oberli A, Slater LM, Cutts E, Brand F, Mundwiler-Pachlatko E, Rusch S, Masik MF, Erat MC, Beck HP, Vakonakis I FASEB J. 2014 Oct;28(10):4420-33. doi: 10.1096/fj.14-256057. Epub 2014 Jun 30. PMID:24983468[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Oberli A, Slater LM, Cutts E, Brand F, Mundwiler-Pachlatko E, Rusch S, Masik MF, Erat MC, Beck HP, Vakonakis I. A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 and comigrates to knobs on the host cell surface. FASEB J. 2014 Oct;28(10):4420-33. doi: 10.1096/fj.14-256057. Epub 2014 Jun 30. PMID:24983468 doi:http://dx.doi.org/10.1096/fj.14-256057
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