1j5h
From Proteopedia
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'''Solution Structure of Apo-Neocarzinostatin''' | '''Solution Structure of Apo-Neocarzinostatin''' | ||
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[[Category: Urbaniak, M D.]] | [[Category: Urbaniak, M D.]] | ||
[[Category: Woolfson, D N.]] | [[Category: Woolfson, D N.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:49:44 2008'' | |
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Revision as of 17:49, 2 May 2008
Solution Structure of Apo-Neocarzinostatin
Overview
The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.
About this Structure
1J5H is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore., Urbaniak MD, Muskett FW, Finucane MD, Caddick S, Woolfson DN, Biochemistry. 2002 Oct 1;41(39):11731-9. PMID:12269815 Page seeded by OCA on Fri May 2 20:49:44 2008
