8dqk
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Intermediate resolution structure of barley (1,3;1,4)-beta-glucan synthase CslF6.== | |
| + | <StructureSection load='8dqk' size='340' side='right'caption='[[8dqk]], [[Resolution|resolution]] 4.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8dqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQK FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dqk OCA], [https://pdbe.org/8dqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dqk RCSB], [https://www.ebi.ac.uk/pdbsum/8dqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dqk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B1P2T4_HORVU B1P2T4_HORVU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mixed-linkage (1,3;1,4)-beta-glucans, which are widely distributed in cell walls of the grasses, are linear glucose polymers containing predominantly (1,4)-beta-linked glucosyl units interspersed with single (1,3)-beta-linked glucosyl units. Their distribution in cereal grains and unique structures are important determinants of dietary fibers that are beneficial to human health. We demonstrate that the barley cellulose synthase-like CslF6 enzyme is sufficient to synthesize a high-molecular weight (1,3;1,4)-beta-glucan in vitro. Biochemical and cryo-electron microscopy analyses suggest that CslF6 functions as a monomer. A conserved "switch motif" at the entrance of the enzyme's transmembrane channel is critical to generate (1,3)-linkages. There, a single-point mutation markedly reduces (1,3)-linkage formation, resulting in the synthesis of cellulosic polysaccharides. Our results suggest that CslF6 monitors the orientation of the nascent polysaccharide's second or third glucosyl unit. Register-dependent interactions with these glucosyl residues reposition the polymer's terminal glucosyl unit to form either a (1,3)- or (1,4)-beta-linkage. | ||
| - | + | Mechanism of mixed-linkage glucan biosynthesis by barley cellulose synthase-like CslF6 (1,3;1,4)-beta-glucan synthase.,Purushotham P, Ho R, Yu L, Fincher GB, Bulone V, Zimmer J Sci Adv. 2022 Nov 11;8(45):eadd1596. doi: 10.1126/sciadv.add1596. Epub 2022 Nov , 11. PMID:36367939<ref>PMID:36367939</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8dqk" style="background-color:#fffaf0;"></div> |
| - | [[Category: Purushotham | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hordeum vulgare]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ho R]] | ||
| + | [[Category: Purushotham P]] | ||
| + | [[Category: Zimmer J]] | ||
Revision as of 10:46, 30 November 2022
Intermediate resolution structure of barley (1,3;1,4)-beta-glucan synthase CslF6.
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