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| | <StructureSection load='4k7t' size='340' side='right'caption='[[4k7t]], [[Resolution|resolution]] 1.10Å' scene=''> | | <StructureSection load='4k7t' size='340' side='right'caption='[[4k7t]], [[Resolution|resolution]] 1.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4k7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K7T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4k7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K7T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPP:GERANYL+DIPHOSPHATE'>GPP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAS:D-ASPARTIC+ACID'>DAS</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=DPN:D-PHENYLALANINE'>DPN</scene>, <scene name='pdbligand=GPP:GERANYL+DIPHOSPHATE'>GPP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ORD:D-ORNITHINE'>ORD</scene>, <scene name='pdbligand=PRD_001070:Bacitracin+A1'>PRD_001070</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAS:D-ASPARTIC+ACID'>DAS</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=DPN:D-PHENYLALANINE'>DPN</scene>, <scene name='pdbligand=ORD:D-ORNITHINE'>ORD</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k7t OCA], [https://pdbe.org/4k7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k7t RCSB], [https://www.ebi.ac.uk/pdbsum/4k7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k7t ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k7t OCA], [http://pdbe.org/4k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k7t RCSB], [http://www.ebi.ac.uk/pdbsum/4k7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k7t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Bacillus subtilis]] | | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Economou, N J]] | + | [[Category: Economou NJ]] |
| - | [[Category: Loll, P J]] | + | [[Category: Loll PJ]] |
| - | [[Category: Antibiotic]]
| + | |
| - | [[Category: Bacitracin]]
| + | |
| - | [[Category: Cell-wall biosynthesis inhibitor]]
| + | |
| - | [[Category: Undecaprenyl-pyrophosphate]]
| + | |
| Structural highlights
4k7t is a 1 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Bacitracin is a metalloantibiotic agent that is widely used as a medicine and feed additive. It interferes with bacterial cell-wall biosynthesis by binding undecaprenyl-pyrophosphate, a lipid carrier that serves as a critical intermediate in cell wall production. Despite bacitracin's broad use, the molecular details of its target recognition have not been elucidated. Here we report a crystal structure for the ternary complex of bacitracin A, zinc, and a geranyl-pyrophosphate ligand at a resolution of 1.1 A. The antibiotic forms a compact structure that completely envelopes the ligand's pyrophosphate group, together with flanking zinc and sodium ions. The complex adopts a highly amphipathic conformation that offers clues to antibiotic function in the context of bacterial membranes. Bacitracin's efficient sequestration of its target represents a previously unseen mode for the recognition of lipid pyrophosphates, and suggests new directions for the design of next-generation antimicrobial agents.
High-resolution crystal structure reveals molecular details of target recognition by bacitracin.,Economou NJ, Cocklin S, Loll PJ Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14207-12. doi:, 10.1073/pnas.1308268110. Epub 2013 Aug 12. PMID:23940351[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Economou NJ, Cocklin S, Loll PJ. High-resolution crystal structure reveals molecular details of target recognition by bacitracin. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14207-12. doi:, 10.1073/pnas.1308268110. Epub 2013 Aug 12. PMID:23940351 doi:10.1073/pnas.1308268110
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