4kfw

From Proteopedia

(Difference between revisions)

Revision as of 11:56, 30 November 2022

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Structural highlights

4kfw is a 2 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GORS2_RAT Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed:10487747, PubMed:23940043). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (By similarity). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB:Q99JX3][UniProtKB:Q9H8Y8]^[1] ^[2]

Publication Abstract from PubMed

The stacking of Golgi cisternae involves GRASP65 and GRASP55. The oligomerization of the N-terminal GRASP domain of these proteins, which consists of two tandem PDZ domains, is required to tether the Golgi membranes. However, the molecular basis for GRASP assembly is unclear. Here, we determined the crystal structures of the GRASP domain of GRASP65 and GRASP55. The structures reveal similar homotypic interactions: the GRASP domain forms a dimer in which the peptide-binding pockets of the two neighboring PDZ2 domains face each other, and the dimers are further connected by the C-terminal tail of one GRASP domain inserting into the binding pocket of the PDZ1 domain in another dimer. Biochemical analysis suggests that both types of contacts are relatively weak but are needed in combination for GRASP-mediated Golgi stacking. Our results unveil a novel mode of membrane tethering by GRASP proteins and provide insight into the mechanism of Golgi stacking.

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins.,Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA. GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system. EMBO J. 1999 Sep 15;18(18):4949-60. PMID:10487747 doi:http://dx.doi.org/10.1093/emboj/18.18.4949
↑ Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins. J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043 doi:http://dx.doi.org/10.1074/jbc.M113.478024
↑ Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins. J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043 doi:http://dx.doi.org/10.1074/jbc.M113.478024

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kfw"

Categories: Large Structures | Rattus norvegicus | Hu J | Liu X

@@ Line 1: / Line 1: @@
 ==Structural insight into Golgi membrane stacking by GRASP65 and GRASP55==
-<StructureSection load='4kfw' size='340' side='right' caption='[[4kfw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='4kfw' size='340' side='right'caption='[[4kfw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4kfw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KFW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4kfw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KFW FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kfv|4kfv]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfw OCA], [https://pdbe.org/4kfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kfw RCSB], [https://www.ebi.ac.uk/pdbsum/4kfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfw ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gorasp2, rCG_26651 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfw OCA], [http://pdbe.org/4kfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kfw RCSB], [http://www.ebi.ac.uk/pdbsum/4kfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GORS2_RAT GORS2_RAT] Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed:10487747, PubMed:23940043). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (By similarity). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB:Q99JX3][UniProtKB:Q9H8Y8]<ref>PMID:10487747</ref> <ref>PMID:23940043</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Buffalo rat]]
+[[Category: Large Structures]]
-[[Category: Hu, J]]
+[[Category: Rattus norvegicus]]
-[[Category: Liu, X]]
+[[Category: Hu J]]
-[[Category: Golgi stacking protein]]
+[[Category: Liu X]]
-[[Category: Signaling protein]]

4kfw

From Proteopedia

Revision as of 11:56, 30 November 2022

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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