7kqv

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==Crystal Structure of aldehyde dehydrogenase (ChALDH) from Cladosporium herbarum==
==Crystal Structure of aldehyde dehydrogenase (ChALDH) from Cladosporium herbarum==
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<StructureSection load='7kqv' size='340' side='right'caption='[[7kqv]]' scene=''>
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<StructureSection load='7kqv' size='340' side='right'caption='[[7kqv]], [[Resolution|resolution]] 3.18&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KQV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7kqv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cladosporium_herbarum Cladosporium herbarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KQV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kqv OCA], [https://pdbe.org/7kqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kqv RCSB], [https://www.ebi.ac.uk/pdbsum/7kqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kqv ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kqv OCA], [https://pdbe.org/7kqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kqv RCSB], [https://www.ebi.ac.uk/pdbsum/7kqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kqv ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALDH_DAVTA ALDH_DAVTA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many protein families have numerous members listed in databases as allergens; however, some allergen database entries, herein called "orphan allergens", are members of large families of which all other members are not allergens. These orphan allergens provide an opportunity to assess whether specific structural features render a protein allergenic. Three orphan allergens [Cladosporium herbarum aldehyde dehydrogenase (ChALDH), Alternaria alternata ALDH (AaALDH), and C. herbarum mannitol dehydrogenase (ChMDH)] were recombinantly produced and purified for structure characterization and for clinical skin prick testing (SPT) in mold allergic participants. Examination of the X-ray crystal structures of ChALDH and ChMDH and a homology structure model of AaALDH did not identify any discernable epitopes that distinguish these putative orphan allergens from their non-allergenic protein relatives. SPT results were aligned with ChMDH being an allergen, 53% of the participants were SPT (+). AaALDH did not elicit SPT reactivity above control proteins not in allergen databases (i.e., Psedomonas syringae indole-3-acetaldehyde dehydrogenase and Zea mays ALDH). Although published results showed consequential human IgE reactivity with ChALDH, no SPT reactivity was observed in this study. With only one of these three orphan allergens, ChMDH, eliciting SPT(+) reactions consistent with the protein being included in allergen databases, this underscores the complicated nature of how bioinformatics is used to assess the potential allergenicity of food proteins that could be newly added to human diets and, when needed, the subsequent clinical testing of that bioinformatic assessment.Trial registration number and date of registration AAC-2017-0467, approved as WIRB protocol #20172536 on 07DEC2017 by WIRB-Copernicus (OHRP/FDA Registration #: IRB00000533, organization #: IORG0000432).
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Biochemical and clinical studies of putative allergens to assess what distinguishes them from other non-allergenic proteins in the same family.,Glenn KC, Silvanovich A, Lee SG, Allen A, Park S, Dunn SE, Kessenich C, Meng C, Vicini JL, Jez JM Transgenic Res. 2022 Oct;31(4-5):507-524. doi: 10.1007/s11248-022-00316-8. Epub , 2022 Aug 8. PMID:35939227<ref>PMID:35939227</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7kqv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cladosporium herbarum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Jez JM]]
[[Category: Jez JM]]
[[Category: Lee SG]]
[[Category: Lee SG]]

Revision as of 08:28, 7 December 2022

Crystal Structure of aldehyde dehydrogenase (ChALDH) from Cladosporium herbarum

PDB ID 7kqv

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