7rn3
From Proteopedia
(Difference between revisions)
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<StructureSection load='7rn3' size='340' side='right'caption='[[7rn3]]' scene=''> | <StructureSection load='7rn3' size='340' side='right'caption='[[7rn3]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[7rn3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Afrohybanthus_enneaspermus Afrohybanthus enneaspermus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RN3 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rn3 OCA], [https://pdbe.org/7rn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rn3 RCSB], [https://www.ebi.ac.uk/pdbsum/7rn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rn3 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rn3 OCA], [https://pdbe.org/7rn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rn3 RCSB], [https://www.ebi.ac.uk/pdbsum/7rn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rn3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CYHED_AFREN CYHED_AFREN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyclotides have a wide range of bioactivities relevant for agricultural and pharmaceutical applications. This large family of naturally occurring macrocyclic peptides is divided into three subfamilies, with the bracelet subfamily being the largest and comprising the most potent cyclotides reported to date. However, attempts to harness the natural bioactivities of bracelet cyclotides and engineer-optimized analogs have been hindered by a lack of understanding of the structural and functional role of their constituent residues, which has been challenging because bracelet cyclotides are difficult to produce synthetically. We recently established a facile strategy to make the I11L mutant of cyclotide hyen D that is as active as the parent peptide, enabling the subsequent production of a series of variants. In the current study, we report an alanine mutagenesis structure-activity study of [I11L] hyen D to probe the role of individual residues on peptide folding using analytical chromatography, on molecular function using surface plasmon resonance, and on therapeutic potential using cytotoxicity assays. We found that Glu-6 and Thr-15 are critical for maintaining the structure of bracelet cyclotides and that hydrophobic residues in loops 2 and 3 are essential for membrane binding and cytotoxic activity, findings that are distinct from the structural and functional characteristics determined for other cyclotide subfamilies. In conclusion, this is the first report of a mutagenesis scan conducted on a bracelet cyclotide, offering insights into their function and supporting future efforts to engineer bracelet cyclotides for biotechnological applications. | ||
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| + | Mutagenesis of bracelet cyclotide hyen D reveals functionally and structurally critical residues for membrane binding and cytotoxicity.,Du Q, Huang YH, Wang CK, Kaas Q, Craik DJ J Biol Chem. 2022 Apr;298(4):101822. doi: 10.1016/j.jbc.2022.101822. Epub 2022 , Mar 11. PMID:35283188<ref>PMID:35283188</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7rn3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Afrohybanthus enneaspermus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Craik DJ]] | [[Category: Craik DJ]] | ||
Current revision
hyen D solution structure
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