1hry

From Proteopedia

---

Revision as of 15:15, 12 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1hry Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

THE 3D STRUCTURE OF THE HUMAN SRY-DNA COMPLEX SOLVED BY MULTID-DIMENSIONAL HETERONUCLEAR-EDITED AND-FILTERED NMR

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The solution structure of the specific complex between the high mobility, group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human, testis-determining gene, and its DNA target site in the promoter of the, mullerian inhibitory substance gene has been determined by, multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that, presents a concave surface (made up of three helices and the N- and, C-terminal strands) to the DNA for sequence-specific recognition. Binding, of hSRY-HMG to its specific target site occurs exclusively in the minor, groove and induces a large conformational change in the DNA. The DNA in, the complex has an overall 70 degrees-80 degrees bend and is helically, unwound relative to classical A- and B-DNA. The structure of the complex, reveals the origin of sequence-specific binding within the HMG-1/HMG-2, family and provides a framework for understanding the effects of point, mutations that cause 46X,Y sex reversal at the atomic level.

Disease

Known diseases associated with this structure: Gonadal dysgenesis, XY type OMIM:[480000]

About this Structure

1HRY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex., Werner MH, Huth JR, Gronenborn AM, Clore GM, Cell. 1995 Jun 2;81(5):705-14. PMID:7774012

Page seeded by OCA on Mon Nov 12 17:21:40 2007