This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1hrz
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1hrz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hrz" /> '''THE 3D STRUCTURE OF THE HUMAN SRY-DNA COMPL...)
Next diff →
Revision as of 15:15, 12 November 2007
|
THE 3D STRUCTURE OF THE HUMAN SRY-DNA COMPLEX SOLVED BY MULTI-DIMENSIONAL HETERONUCLEAR-EDITED AND-FILTERED NMR
Contents |
Overview
The solution structure of the specific complex between the high mobility, group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human, testis-determining gene, and its DNA target site in the promoter of the, mullerian inhibitory substance gene has been determined by, multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that, presents a concave surface (made up of three helices and the N- and, C-terminal strands) to the DNA for sequence-specific recognition. Binding, of hSRY-HMG to its specific target site occurs exclusively in the minor, groove and induces a large conformational change in the DNA. The DNA in, the complex has an overall 70 degrees-80 degrees bend and is helically, unwound relative to classical A- and B-DNA. The structure of the complex, reveals the origin of sequence-specific binding within the HMG-1/HMG-2, family and provides a framework for understanding the effects of point, mutations that cause 46X,Y sex reversal at the atomic level.
Disease
Known diseases associated with this structure: Gonadal dysgenesis, XY type OMIM:[480000]
About this Structure
1HRZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex., Werner MH, Huth JR, Gronenborn AM, Clore GM, Cell. 1995 Jun 2;81(5):705-14. PMID:7774012
Page seeded by OCA on Mon Nov 12 17:21:43 2007
