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| | ==Crystal Structure of MyoVa-GTD in Complex with Two Cargos== | | ==Crystal Structure of MyoVa-GTD in Complex with Two Cargos== |
| - | <StructureSection load='4kp3' size='340' side='right' caption='[[4kp3]], [[Resolution|resolution]] 2.41Å' scene=''> | + | <StructureSection load='4kp3' size='340' side='right'caption='[[4kp3]], [[Resolution|resolution]] 2.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4kp3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KP3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4kp3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KP3 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wb8|3wb8]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kp3 OCA], [https://pdbe.org/4kp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kp3 RCSB], [https://www.ebi.ac.uk/pdbsum/4kp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kp3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Myo5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Rilpl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Mlph ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kp3 OCA], [http://pdbe.org/4kp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kp3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kp3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYO5A_MOUSE MYO5A_MOUSE]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:22908308</ref> [[http://www.uniprot.org/uniprot/MELPH_MOUSE MELPH_MOUSE]] Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.<ref>PMID:11887186</ref> [[http://www.uniprot.org/uniprot/RIPL2_MOUSE RIPL2_MOUSE]] Involved in cell shape and neuronal morphogenesis, positively regulating the establishment and maintenance of dendritic spines. May activate RAC1 (By similarity). | + | [https://www.uniprot.org/uniprot/MYO5A_MOUSE MYO5A_MOUSE] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:22908308</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4kp3" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4kp3" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Exophilin 3D structures|Exophilin 3D structures]] |
| | + | *[[Myosin 3D Structures|Myosin 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Liu, X]] | + | [[Category: Mus musculus]] |
| - | [[Category: Wei, Z]] | + | [[Category: Liu X]] |
| - | [[Category: Yu, C]] | + | [[Category: Wei Z]] |
| - | [[Category: Zhang, M]] | + | [[Category: Yu C]] |
| - | [[Category: Helix bundle]]
| + | [[Category: Zhang M]] |
| - | [[Category: Motor protein-protein transport complex]]
| + | |
| Structural highlights
Function
MYO5A_MOUSE Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.[1]
Publication Abstract from PubMed
Class V myosins (MyoV), the most studied unconventional myosins, recognize numerous cargos mainly via the motor's globular tail domain (GTD). Little is known regarding how MyoV-GTD recognizes such a diverse array of cargos specifically. Here, we solved the crystal structures of MyoVa-GTD in its apo-form and in complex with two distinct cargos, melanophilin and Rab interacting lysosomal protein-like 2. The apo-MyoVa-GTD structure indicates that most mutations found in patients with Griscelli syndrome, microvillus inclusion disease, or cancers or in "dilute" rodents likely impair the folding of GTD. The MyoVa-GTD/cargo complex structure reveals two distinct cargo-binding surfaces, one primarily via charge-charge interaction and the other mainly via hydrophobic interactions. Structural and biochemical analysis reveal the specific cargo-binding specificities of various isoforms of mammalian MyoV as well as very different cargo recognition mechanisms of MyoV between yeast and higher eukaryotes. The MyoVa-GTD structures resolved here provide a framework for future functional studies of vertebrate class V myosins.
Structural basis of cargo recognitions for class V myosins.,Wei Z, Liu X, Yu C, Zhang M Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798443[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chen Y, Wang Y, Zhang J, Deng Y, Jiang L, Song E, Wu XS, Hammer JA, Xu T, Lippincott-Schwartz J. Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes. J Cell Biol. 2012 Aug 20;198(4):545-60. doi: 10.1083/jcb.201111091. PMID:22908308 doi:10.1083/jcb.201111091
- ↑ Wei Z, Liu X, Yu C, Zhang M. Structural basis of cargo recognitions for class V myosins. Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798443 doi:10.1073/pnas.1306768110
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