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| | ==Structure of GrlR-GrlA complex== | | ==Structure of GrlR-GrlA complex== |
| - | <StructureSection load='4kt5' size='340' side='right' caption='[[4kt5]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4kt5' size='340' side='right'caption='[[4kt5]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4kt5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KT5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4kt5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KT5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt5 OCA], [https://pdbe.org/4kt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kt5 RCSB], [https://www.ebi.ac.uk/pdbsum/4kt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt5 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">grlR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), grlA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt5 OCA], [http://pdbe.org/4kt5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kt5 RCSB], [http://www.ebi.ac.uk/pdbsum/4kt5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7DB61_ECO57 Q7DB61_ECO57] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco57]] | + | [[Category: Escherichia coli O157:H7]] |
| - | [[Category: Jobichen, C]]
| + | [[Category: Large Structures]] |
| - | [[Category: Padavannil, A]] | + | [[Category: Jobichen C]] |
| - | [[Category: Sivaraman, J]] | + | [[Category: Padavannil A]] |
| - | [[Category: Beta barrel]] | + | [[Category: Sivaraman J]] |
| - | [[Category: Hth motif]] | + | |
| - | [[Category: Regulator]]
| + | |
| - | [[Category: Transcription regulator]]
| + | |
| Structural highlights
Function
Q7DB61_ECO57
Publication Abstract from PubMed
The locus of enterocyte effacement (LEE) is essential for virulence of enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). The 41 genes of the LEE encode type III secretion system proteins and three associated regulators: Ler, GrlA and GrlR. Ler is a positive regulator for most of the LEE operons, including grlRA. GrlA controls the expression of ler, ehxCABD and flhDC operons. GrlR binds to GrlA and suppresses its function. Here we report the crystal structure of GrlR-GrlADelta (aa 1-106) complex (2:1) and its functional characterization. We show that GrlR interacts with the Helix-Turn-Helix motif of GrlA. Moreover, GrlA binds to the promoter DNA fragments of ler, ehxCABD and flhDC, and GrlR outcompetes with these promoter DNA sequences for the Helix-Turn-Helix motif of GrlA. These findings provide mechanistic insight into a regulatory module for the virulence of EPEC and EHEC, two important pathogens that cause devastating diseases.
Structure of GrlR-GrlA complex that prevents GrlA activation of virulence genes.,Padavannil A, Jobichen C, Mills E, Velazquez-Campoy A, Li M, Leung KY, Mok YK, Rosenshine I, Sivaraman J Nat Commun. 2013 Oct 4;4:2546. doi: 10.1038/ncomms3546. PMID:24092262[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Padavannil A, Jobichen C, Mills E, Velazquez-Campoy A, Li M, Leung KY, Mok YK, Rosenshine I, Sivaraman J. Structure of GrlR-GrlA complex that prevents GrlA activation of virulence genes. Nat Commun. 2013 Oct 4;4:2546. doi: 10.1038/ncomms3546. PMID:24092262 doi:http://dx.doi.org/10.1038/ncomms3546
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