|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of a type VI secretion system effector-immunity complex from Pseudomonas protegens== | | ==Structure of a type VI secretion system effector-immunity complex from Pseudomonas protegens== |
| - | <StructureSection load='4kt3' size='340' side='right' caption='[[4kt3]], [[Resolution|resolution]] 1.44Å' scene=''> | + | <StructureSection load='4kt3' size='340' side='right'caption='[[4kt3]], [[Resolution|resolution]] 1.44Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4kt3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psef5 Psef5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KT3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4kt3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KT3 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFL_3037 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5]), PFL_3036 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt3 OCA], [https://pdbe.org/4kt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kt3 RCSB], [https://www.ebi.ac.uk/pdbsum/4kt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt3 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt3 OCA], [http://pdbe.org/4kt3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kt3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kt3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q4KC90_PSEF5 Q4KC90_PSEF5] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Psef5]] | + | [[Category: Large Structures]] |
| - | [[Category: Chou, S]] | + | [[Category: Pseudomonas protegens Pf-5]] |
| - | [[Category: Gardiner, T E]] | + | [[Category: Chou S]] |
| - | [[Category: Mougous, J D]] | + | [[Category: Gardiner TE]] |
| - | [[Category: Whitney, J C]] | + | [[Category: Mougous JD]] |
| - | [[Category: Glycoside hydrolase]] | + | [[Category: Whitney JC]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pfl_3036]]
| + | |
| Structural highlights
Function
Q4KC90_PSEF5
Publication Abstract from PubMed
Bacteria employ type VI secretion systems (T6SSs) to facilitate antagonistic interactions towards prokaryotic and eukaryotic cells. Despite the widespread identification of T6SSs among Gram-negative bacteria, the number of experimentally validated substrate effector proteins mediating these interactions remains small. Here, employing an informatics approach, we define novel families of T6S peptidoglycan glycoside hydrolase effectors. Consistent with the known intercellular selfintoxication exhibited by the T6S pathway, we observe that each effector gene is located adjacent to a hypothetical open reading frame encoding a putative periplasmically-localized immunity determinant. To validate our sequence-based approach, we functionally investigate a representative family member from the soil-dwelling bacterium Pseudomonas protegens. We demonstrate that this protein is secreted in a T6SS-dependent manner and that it confers a fitness advantage in growth competition assays with Pseudomonas putida. In addition, we determined the 1.4 A X-ray crystal structure of this effector in complex with its cognate immunity protein. The structure reveals the effector shares highest overall structural similarity to a glycoside hydrolase family associated with peptidoglycan Nacetylglucosaminidase activity - suggesting that T6S peptidoglycan glycoside hydrolase effector families may comprise significant enzymatic diversity. Our structural analyses also demonstrate that self-intoxication is prevented by direct occlusion of the active site in a manner that would prevent binding of the murein substrate. This work significantly expands our current understanding of T6S effector diversity.
Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair.,Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD J Biol Chem. 2013 Jul 22. PMID:23878199[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD. Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. J Biol Chem. 2013 Jul 22. PMID:23878199 doi:10.1074/jbc.M113.488320
|
