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| | ==Lactococcus phage 67 RuvC - apo form== | | ==Lactococcus phage 67 RuvC - apo form== |
| - | <StructureSection load='4ktw' size='340' side='right' caption='[[4ktw]], [[Resolution|resolution]] 1.68Å' scene=''> | + | <StructureSection load='4ktw' size='340' side='right'caption='[[4ktw]], [[Resolution|resolution]] 1.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ktw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_bil67 Bacteriophage bil67]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ktw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_virus_bIL67 Lactococcus virus bIL67]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KTW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ktz|4ktz]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ktw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktw OCA], [https://pdbe.org/4ktw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ktw RCSB], [https://www.ebi.ac.uk/pdbsum/4ktw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktw ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36343 Bacteriophage bIL67])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktw OCA], [http://pdbe.org/4ktw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ktw RCSB], [http://www.ebi.ac.uk/pdbsum/4ktw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q38242_9CAUD Q38242_9CAUD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacteriophage bil67]] | + | [[Category: Lactococcus virus bIL67]] |
| - | [[Category: Green, V]] | + | [[Category: Large Structures]] |
| - | [[Category: Rafferty, J B]] | + | [[Category: Green V]] |
| - | [[Category: Dna junction endonuclease]] | + | [[Category: Rafferty JB]] |
| - | [[Category: Dna junction]]
| + | |
| - | [[Category: Endonuclease]]
| + | |
| - | [[Category: Holliday junction]]
| + | |
| - | [[Category: Holliday junctions and other 2- & 3-way dna junction]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Phage packaging]]
| + | |
| - | [[Category: Recombination]]
| + | |
| - | [[Category: Replication]]
| + | |
| - | [[Category: Rnase-h fold]]
| + | |
| Structural highlights
Function
Q38242_9CAUD
Publication Abstract from PubMed
Viral and bacterial Holliday junction resolvases differ in specificity with the former typically being more promiscuous, acting on a variety of branched DNA substrates, while the latter exclusively targets Holliday junctions. We have determined the crystal structure of a RuvC resolvase from bacteriophage bIL67 to help identify features responsible for DNA branch discrimination. Comparisons between phage and bacterial RuvC structures revealed significant differences in the number and position of positively-charged residues in the outer sides of the junction binding cleft. Substitutions were generated in phage RuvC residues implicated in branch recognition and six were found to confer defects in Holliday junction and replication fork cleavage in vivo. Two mutants, R121A and R124A that flank the DNA binding site were purified and exhibited reduced in vitro binding to fork and linear duplex substrates relative to the wild-type, while retaining the ability to bind X junctions. Crucially, these two variants cleaved Holliday junctions with enhanced specificity and symmetry, a feature more akin to cellular RuvC resolvases. Thus, additional positive charges in the phage RuvC binding site apparently stabilize productive interactions with branched structures other than the canonical Holliday junction, a feature advantageous for viral DNA processing but deleterious for their cellular counterparts.
Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry.,Green V, Curtis FA, Sedelnikova S, Rafferty JB, Sharples GJ Mol Microbiol. 2013 Jul 25. doi: 10.1111/mmi.12343. PMID:23888987[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Green V, Curtis FA, Sedelnikova S, Rafferty JB, Sharples GJ. Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry. Mol Microbiol. 2013 Jul 25. doi: 10.1111/mmi.12343. PMID:23888987 doi:10.1111/mmi.12343
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