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| | ==Crystal structure of Thermus thermophilus CarD== | | ==Crystal structure of Thermus thermophilus CarD== |
| - | <StructureSection load='4l5g' size='340' side='right' caption='[[4l5g]], [[Resolution|resolution]] 2.39Å' scene=''> | + | <StructureSection load='4l5g' size='340' side='right'caption='[[4l5g]], [[Resolution|resolution]] 2.39Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4l5g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L5G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4l5g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L5G FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CarD, TTHA0168 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l5g OCA], [https://pdbe.org/4l5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l5g RCSB], [https://www.ebi.ac.uk/pdbsum/4l5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l5g ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l5g OCA], [http://pdbe.org/4l5g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l5g RCSB], [http://www.ebi.ac.uk/pdbsum/4l5g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l5g ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5SLX5_THET8 Q5SLX5_THET8] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet8]] | + | [[Category: Large Structures]] |
| - | [[Category: Campbell, E A]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Darst, S A]] | + | [[Category: Campbell EA]] |
| - | [[Category: Srivastava, D B]] | + | [[Category: Darst SA]] |
| - | [[Category: Westblade, L F]] | + | [[Category: Srivastava DB]] |
| - | [[Category: Rna polymerase]] | + | [[Category: Westblade LF]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulator]]
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| Structural highlights
Function
Q5SLX5_THET8
Publication Abstract from PubMed
CarD, an essential transcription regulator in Mycobacterium tuberculosis, directly interacts with the RNA polymerase (RNAP). We used a combination of in vivo and in vitro approaches to establish that CarD is a global regulator that stimulates the formation of RNAP-holoenzyme open promoter (RPo) complexes. We determined the X-ray crystal structure of Thermus thermophilus CarD, allowing us to generate a structural model of the CarD/RPo complex. On the basis of our structural and functional analyses, we propose that CarD functions by forming protein/protein and protein/DNA interactions that bridge the RNAP to the promoter DNA. CarD appears poised to interact with a DNA structure uniquely presented by the RPo: the splayed minor groove at the double-stranded/single-stranded DNA junction at the upstream edge of the transcription bubble. Thus, CarD uses an unusual mechanism for regulating transcription, sensing the DNA conformation where transcription bubble formation initiates.
Structure and function of CarD, an essential mycobacterial transcription factor.,Srivastava DB, Leon K, Osmundson J, Garner AL, Weiss LA, Westblade LF, Glickman MS, Landick R, Darst SA, Stallings CL, Campbell EA Proc Natl Acad Sci U S A. 2013 Jul 15. PMID:23858468[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Srivastava DB, Leon K, Osmundson J, Garner AL, Weiss LA, Westblade LF, Glickman MS, Landick R, Darst SA, Stallings CL, Campbell EA. Structure and function of CarD, an essential mycobacterial transcription factor. Proc Natl Acad Sci U S A. 2013 Jul 15. PMID:23858468 doi:10.1073/pnas.1308270110
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