1jax
From Proteopedia
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'''Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)''' | '''Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)''' | ||
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[[Category: Thauer, R.]] | [[Category: Thauer, R.]] | ||
[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
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Revision as of 17:59, 2 May 2008
Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)
Overview
Cofactor F420 is a 5'-deazaflavin derivative first discovered in methanogenic archaea but later found also to be present in some bacteria. As a coenzyme, it is involved in hydride transfer reactions and as a prosthetic group in the DNA photolyase reaction. We report here for the first time on the crystal structure of an F420-dependent oxidoreductase bound with F420. The structure of F420H2:NADP+ oxidoreductase resolved to 1.65 A contains two domains: an N-terminal domain characteristic of a dinucleotide-binding Rossmann fold and a smaller C-terminal domain. The nicotinamide and the deazaflavin part of the two coenzymes are bound in the cleft between the domains such that the Si-faces of both face each other at a distance of 3.1 A, which is optimal for hydride transfer. Comparison of the structures bound with and without substrates reveals that of the two substrates NADP has to bind first, the binding being associated with an induced fit.
About this Structure
1JAX is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound., Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer RK, Iwata M, Iwata S, Ermler U, Shima S, EMBO J. 2001 Dec 3;20(23):6561-9. PMID:11726492 Page seeded by OCA on Fri May 2 20:59:49 2008
