1jbt

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[[Image:1jbt.gif|left|200px]]
[[Image:1jbt.gif|left|200px]]
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{{Structure
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|PDB= 1jbt |SIZE=350|CAPTION= <scene name='initialview01'>1jbt</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1jbt", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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{{STRUCTURE_1jbt| PDB=1jbt | SCENE= }}
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|RELATEDENTRY=[[1aqz|1AQZ]], [[430d|430D]], [[480d|480D]], [[483d|483D]], [[1jbr|1JBR]], [[1jbs|1JBS]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbt OCA], [http://www.ebi.ac.uk/pdbsum/1jbt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jbt RCSB]</span>
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'''CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG'''
'''CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG'''
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[[Category: Glover, L.]]
[[Category: Glover, L.]]
[[Category: Yang, X.]]
[[Category: Yang, X.]]
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[[Category: highly specific ribonuclease]]
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[[Category: Highly specific ribonuclease]]
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[[Category: protein-rna recognition]]
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[[Category: Protein-rna recognition]]
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[[Category: restrictocin]]
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[[Category: Restrictocin]]
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[[Category: ribonuclease t1]]
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[[Category: Ribonuclease t1]]
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[[Category: ribotoxin]]
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[[Category: Ribotoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:01:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:23 2008''
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Revision as of 18:01, 2 May 2008

Image:1jbt.gif

Template:STRUCTURE 1jbt

CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG


Overview

The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.

About this Structure

1JBT is a Single protein structure of sequence from Aspergillus restrictus. Full crystallographic information is available from OCA.

Reference

Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping., Yang X, Gerczei T, Glover LT, Correll CC, Nat Struct Biol. 2001 Nov;8(11):968-73. PMID:11685244 Page seeded by OCA on Fri May 2 21:01:55 2008

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