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| | <StructureSection load='4lhe' size='340' side='right'caption='[[4lhe]], [[Resolution|resolution]] 1.96Å' scene=''> | | <StructureSection load='4lhe' size='340' side='right'caption='[[4lhe]], [[Resolution|resolution]] 1.96Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lhe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bac25 Bac25]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LHE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lhe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._H-257 Bacillus sp. H-257]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylglycerol_lipase Acylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.23 3.1.1.23] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhe OCA], [https://pdbe.org/4lhe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lhe RCSB], [https://www.ebi.ac.uk/pdbsum/4lhe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhe ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhe OCA], [http://pdbe.org/4lhe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lhe RCSB], [http://www.ebi.ac.uk/pdbsum/4lhe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhe ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MGLP_BAC25 MGLP_BAC25]] Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol. | + | [https://www.uniprot.org/uniprot/MGLP_BAC25 MGLP_BAC25] Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Lipase|Lipase]] | + | *[[Lipase 3D Structures|Lipase 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acylglycerol lipase]] | + | [[Category: Bacillus sp. H-257]] |
| - | [[Category: Bac25]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tsuge, H]] | + | [[Category: Tsuge H]] |
| - | [[Category: Tsurumura, T]] | + | [[Category: Tsurumura T]] |
| - | [[Category: Alpha/beta hydrolase fold]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
MGLP_BAC25 Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol.
Publication Abstract from PubMed
Lipases, which are conserved from bacteria to mammals, catalyze the hydrolysis of acylglycerol to free fatty acids and glycerol. Monoacylglycerol lipase (MGL) specifically catalyzes the hydrolysis of monoacylglycerol. Although there have been numerous studies of the structure of lipases, there have been few studies of MGL. Here, we report the crystal structure of authentic MGL isolated from Bacillus sp. H257 (bMGL). The crystal diffracts to 1.96 A resolution. It belongs to space group P21212, and the unit cell parameters are a = 99.7 A, b = 106.1 A and c = 43.0 A. As in other lipases, three structural features for lipase activity are conserved in bMGL: the glycine-X-serine-X-glycine motif, catalytic triad and cap region. The structure of bMGL appears to be closed, as the cap region covers the active site entrance. The isolated bMGL hydrolyzed 2-AG, a known human MGL-specific substrate. Based on a 2-AG bound model, we discuss the substrate selectivity. The functional and structural features of bMGL provide insight how its substrate selectivity is determined and how specific inhibitors of bacterial MGL could be designed, which may be useful for development of novel antibiotics.
Substrate selectivity of bacterial monoacylglycerol lipase based on crystal structure.,Tsurumura T, Tsuge H J Struct Funct Genomics. 2014 Jun 4. PMID:24894647[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tsurumura T, Tsuge H. Substrate selectivity of bacterial monoacylglycerol lipase based on crystal structure. J Struct Funct Genomics. 2014 Jun 4. PMID:24894647 doi:http://dx.doi.org/10.1007/s10969-014-9181-2
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