|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4lku' size='340' side='right'caption='[[4lku]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='4lku' size='340' side='right'caption='[[4lku]], [[Resolution|resolution]] 1.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lku]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LKU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LKU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lku]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LKU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lku OCA], [http://pdbe.org/4lku PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lku RCSB], [http://www.ebi.ac.uk/pdbsum/4lku PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lku ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lku OCA], [https://pdbe.org/4lku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lku RCSB], [https://www.ebi.ac.uk/pdbsum/4lku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lku ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/C9QXJ2_ECOD1 C9QXJ2_ECOD1]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).[SAAS:SAAS001185_004_016395][HAMAP-Rule:MF_00115] | + | [https://www.uniprot.org/uniprot/MSCL_ECOLI MSCL_ECOLI] Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.<ref>PMID:10202137</ref> <ref>PMID:23416054</ref> <ref>PMID:23875651</ref> <ref>PMID:7511799</ref> <ref>PMID:9632260</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 24: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Escherichia coli DH1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rees, D C]] | + | [[Category: Rees DC]] |
| - | [[Category: Walton, T A]] | + | [[Category: Walton TA]] |
| - | [[Category: Pentamer]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
MSCL_ECOLI Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.[1] [2] [3] [4] [5]
Publication Abstract from PubMed
The crystal structure of the cytoplasmic domain (CTD) from the mechanosensitive channel of large conductance (MscL) in E. coli has been determined at 1.45 A resolution. This domain forms a pentameric coiled coil similar to that observed in the structure of MscL from M. tuberculosis and also found in the Cartilage Oligomeric Matrix Protein (COMPcc). It contains canonical hydrophobic and atypical ionic interactions compared to previously characterized coiled coil structures. Thermodynamic analysis indicates that while the free EcMscL-CTD is less stable than other coiled coils, it is likely to remain folded in context of the full-length channel.
Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance.,Walton TA, Rees DC Protein Sci. 2013 Aug 29. doi: 10.1002/pro.2360. PMID:24038743[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999 Apr 1;18(7):1730-7. PMID:10202137 doi:http://dx.doi.org/10.1093/emboj/18.7.1730
- ↑ Yang LM, Zhong D, Blount P. Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep. 2013 Feb 21;3(2):520-7. doi: 10.1016/j.celrep.2013.01.018. Epub 2013 , Feb 14. PMID:23416054 doi:http://dx.doi.org/10.1016/j.celrep.2013.01.018
- ↑ Zhong D, Blount P. Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug , 1. PMID:23875651 doi:http://dx.doi.org/10.1021/bi400790j
- ↑ Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994 Mar 17;368(6468):265-8. PMID:7511799 doi:http://dx.doi.org/10.1038/368265a0
- ↑ Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. doi: 10.1046/j.1365-2958.1998.00821.x. PMID:9632260 doi:http://dx.doi.org/10.1046/j.1365-2958.1998.00821.x
- ↑ Walton TA, Rees DC. Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance. Protein Sci. 2013 Aug 29. doi: 10.1002/pro.2360. PMID:24038743 doi:10.1002/pro.2360
|
