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4lnu

From Proteopedia

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Revision as of 11:14, 14 December 2022

Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin

Structural highlights

4lnu is a 4 chain structure with sequence from Homo sapiens, Ovis aries and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

W5QC38_SHEEP Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[ARBA:ARBA00004036][RuleBase:RU000352]

Publication Abstract from PubMed

Kinesin-1 is a dimeric ATP-dependent motor protein that moves towards microtubules (+) ends. This movement is driven by two conformations (docked and undocked) of the two motor domains carboxy-terminal peptides (named neck linkers), in correlation with the nucleotide bound to each motor domain. Despite extensive data on kinesin-1, the structural connection between its nucleotide cycle and movement has remained elusive, mostly because the structure of the critical tubulin-bound apo-kinesin state was unknown. Here we report the 2.2 A structure of this complex. From its comparison with detached kinesin-ADP and tubulin-bound kinesin-ATP, we identify three kinesin motor subdomains that move rigidly along the nucleotide cycle. Our data reveal how these subdomains reorient on binding to tubulin and when ATP binds, leading respectively to ADP release and to neck linker docking. These results establish a framework for understanding the transformation of chemical energy into mechanical work by (+) end-directed kinesins.

The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement.,Cao L, Wang W, Jiang Q, Wang C, Knossow M, Gigant B Nat Commun. 2014 Nov 14;5:5364. doi: 10.1038/ncomms6364. PMID:25395082^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cao L, Wang W, Jiang Q, Wang C, Knossow M, Gigant B. The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement. Nat Commun. 2014 Nov 14;5:5364. doi: 10.1038/ncomms6364. PMID:25395082 doi:http://dx.doi.org/10.1038/ncomms6364

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lnu"

@@ Line 3: / Line 3: @@
 <StructureSection load='4lnu' size='340' side='right'caption='[[4lnu]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4lnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human], [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LNU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4lnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LNU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIF5B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lnu OCA], [https://pdbe.org/4lnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lnu RCSB], [https://www.ebi.ac.uk/pdbsum/4lnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lnu ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lnu OCA], [http://pdbe.org/4lnu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lnu RCSB], [http://www.ebi.ac.uk/pdbsum/4lnu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lnu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN]] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
+[https://www.uniprot.org/uniprot/W5QC38_SHEEP W5QC38_SHEEP] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[ARBA:ARBA00004036][RuleBase:RU000352]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 20: @@
 ==See Also==
-*[[Kinesin|Kinesin]]
+*[[Kinesin 3D Structures|Kinesin 3D Structures]]
-*[[Tubulin|Tubulin]]
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
 [[Category: Ovis aries]]
-[[Category: Synthetic construct sequences]]
+[[Category: Synthetic construct]]
-[[Category: Cao, L]]
+[[Category: Cao L]]
-[[Category: Gigant, B]]
+[[Category: Gigant B]]
-[[Category: Knossow, M]]
+[[Category: Knossow M]]
-[[Category: Alpha-tubulin]]
-[[Category: Apo-kinesin]]
-[[Category: Beta-tubulin]]
-[[Category: Cell cycle-motor protein complex]]
-[[Category: Darpin]]
-[[Category: Kinesin]]
-[[Category: Microtubule]]
-[[Category: Tubulin]]

4lnu

From Proteopedia

Revision as of 11:14, 14 December 2022

Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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