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| | ==HA70-alpha2,3-SiaLC== | | ==HA70-alpha2,3-SiaLC== |
| - | <StructureSection load='4lo5' size='340' side='right' caption='[[4lo5]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4lo5' size='340' side='right'caption='[[4lo5]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lo5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LO5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lo5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LO5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900025:3-sialyl-alpha-lactose'>PRD_900025</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lo5 OCA], [https://pdbe.org/4lo5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lo5 RCSB], [https://www.ebi.ac.uk/pdbsum/4lo5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lo5 ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lo0|4lo0]], [[4lo1|4lo1]], [[4lo2|4lo2]], [[4lo3|4lo3]], [[4lo4|4lo4]], [[4lo6|4lo6]], [[4lo7|4lo7]], [[4lo8|4lo8]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ha70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lo5 OCA], [http://pdbe.org/4lo5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lo5 RCSB], [http://www.ebi.ac.uk/pdbsum/4lo5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lo5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8KHU9_CLOBO Q8KHU9_CLOBO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus botulinus van ermengem 1896]] | + | [[Category: Clostridium botulinum]] |
| - | [[Category: Cheng, L W]] | + | [[Category: Large Structures]] |
| - | [[Category: Gu, S]] | + | [[Category: Cheng LW]] |
| - | [[Category: Jin, L]] | + | [[Category: Gu S]] |
| - | [[Category: Jin, R]] | + | [[Category: Jin L]] |
| - | [[Category: Kruel, A M]] | + | [[Category: Jin R]] |
| - | [[Category: Le, T T]] | + | [[Category: Kruel AM]] |
| - | [[Category: Lee, K]] | + | [[Category: Le TT]] |
| - | [[Category: Perry, K]] | + | [[Category: Lee K]] |
| - | [[Category: Rummel, A]] | + | [[Category: Perry K]] |
| - | [[Category: Strotmeier, J]] | + | [[Category: Rummel A]] |
| - | [[Category: Yao, G]] | + | [[Category: Strotmeier J]] |
| - | [[Category: Botulinum neurotoxin]]
| + | [[Category: Yao G]] |
| - | [[Category: Botulism]]
| + | |
| - | [[Category: Carbohydrate/sugar binding]]
| + | |
| - | [[Category: Hemagglutinin]]
| + | |
| - | [[Category: Neurotoxin associated protein]]
| + | |
| - | [[Category: Progenitor toxin complex]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Secreted protein]]
| + | |
| Structural highlights
Function
Q8KHU9_CLOBO
Publication Abstract from PubMed
Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary proteins as progenitor toxin complexes (PTCs) to become highly potent oral poisons. Here, we report the structure of a approximately 760 kDa 14-subunit large PTC of serotype A (L-PTC/A) and reveal insight into its absorption mechanism. Using a combination of X-ray crystallography, electron microscopy, and functional studies, we found that L-PTC/A consists of two structurally and functionally independent sub-complexes. A hetero-dimeric 290 kDa complex protects BoNT, while a hetero-dodecameric 470 kDa complex facilitates its absorption in the harsh environment of the gastrointestinal tract. BoNT absorption is mediated by nine glycan-binding sites on the dodecameric sub-complex that forms multivalent interactions with carbohydrate receptors on intestinal epithelial cells. We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry.
Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.,Lee K, Gu S, Jin L, Le TT, Cheng LW, Strotmeier J, Kruel AM, Yao G, Perry K, Rummel A, Jin R PLoS Pathog. 2013 Oct;9(10):e1003690. doi: 10.1371/journal.ppat.1003690. Epub, 2013 Oct 10. PMID:24130488[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee K, Gu S, Jin L, Le TT, Cheng LW, Strotmeier J, Kruel AM, Yao G, Perry K, Rummel A, Jin R. Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity. PLoS Pathog. 2013 Oct;9(10):e1003690. doi: 10.1371/journal.ppat.1003690. Epub, 2013 Oct 10. PMID:24130488 doi:http://dx.doi.org/10.1371/journal.ppat.1003690
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