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| | <StructureSection load='4lqk' size='340' side='right'caption='[[4lqk]], [[Resolution|resolution]] 1.99Å' scene=''> | | <StructureSection load='4lqk' size='340' side='right'caption='[[4lqk]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lqk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccw Vaccw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LQK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lqk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LQK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lqk OCA], [https://pdbe.org/4lqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lqk RCSB], [https://www.ebi.ac.uk/pdbsum/4lqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lqk ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VACWR172, A46R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10254 VACCW])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lqk OCA], [http://pdbe.org/4lqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lqk RCSB], [http://www.ebi.ac.uk/pdbsum/4lqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lqk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A46_VACCW A46_VACCW]] Bcl-2-like protein which disrupts the host immune response by inhibiting the TLR4 signaling pathway leading to NF-kappa-B activation. Targets several host TIR adapters including MYD88, TIRAP, TRIF and TICAM2 thereby blocking NF-kappa-B and TRIF-mediated IRF3 activation. | + | [https://www.uniprot.org/uniprot/A46_VACCW A46_VACCW] Bcl-2-like protein which disrupts the host immune response by inhibiting the TLR4 signaling pathway leading to NF-kappa-B activation. Targets several host TIR adapters including MYD88, TIRAP, TRIF and TICAM2 thereby blocking NF-kappa-B and TRIF-mediated IRF3 activation. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vaccw]] | + | [[Category: Vaccinia virus WR]] |
| - | [[Category: Djinovic-Carugo, K]] | + | [[Category: Djinovic-Carugo K]] |
| - | [[Category: Fedosyuk, S]] | + | [[Category: Fedosyuk S]] |
| - | [[Category: Grishkovskaya, I]] | + | [[Category: Grishkovskaya I]] |
| - | [[Category: Skern, T]] | + | [[Category: Skern T]] |
| - | [[Category: Bcl-2-like fold]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
A46_VACCW Bcl-2-like protein which disrupts the host immune response by inhibiting the TLR4 signaling pathway leading to NF-kappa-B activation. Targets several host TIR adapters including MYD88, TIRAP, TRIF and TICAM2 thereby blocking NF-kappa-B and TRIF-mediated IRF3 activation.
Publication Abstract from PubMed
Successful vaccinia virus (VACV) replication in the host requires expression of viral proteins that interfere with host immunity, such as antagonists of the activation of the proinflammatory transcription factor NF-kappaB. Two such VACV proteins are A46 and A52. A46 interacts with the Toll-like receptor/interleukin-1R (TIR) domain of Toll-like receptors and intracellular adaptors such as MAL (MyD88 adapter-like), TRAM (TIR domain-containing adapter-inducing interferon-beta (TRIF)-related adaptor molecule), TRIF, and MyD88, whereas A52 binds to the downstream signaling components TRAF6 and IRAK2. Here, we characterize A46 biochemically, determine by microscale thermophoresis binding constants for the interaction of A46 with the TIR domains of MyD88 and MAL, and present the 2.0 A resolution crystal structure of A46 residues 87-229. Full-length A46 behaves as a tetramer; variants lacking the N-terminal 80 residues are dimeric. Nevertheless, both bind to the Toll-like receptor domains of MAL and MyD88 with KD values in the low mum range. Like A52, A46 also shows a Bcl-2-like fold but with biologically relevant differences from that of A52. Thus, A46 uses helices alpha4 and alpha6 to dimerize, compared with the alpha1-alpha6 face used by A52 and other Bcl-2 like VACV proteins. Furthermore, the loop between A46 helices alpha4-alpha5 is flexible and shorter than in A52; there is also evidence for an intramolecular disulfide bridge between consecutive cysteine residues. We used molecular docking to propose how A46 interacts with the BB loop of the TRAM TIR domain. Comparisons of A46 and A52 exemplify how subtle changes in viral proteins with the same fold lead to crucial differences in biological activity.
Characterization and structure of the vaccinia virus NF-kappaB antagonist A46.,Fedosyuk S, Grishkovskaya I, de Almeida Ribeiro E Jr, Skern T J Biol Chem. 2014 Feb 7;289(6):3749-62. doi: 10.1074/jbc.M113.512756. Epub 2013, Dec 19. PMID:24356965[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fedosyuk S, Grishkovskaya I, de Almeida Ribeiro E Jr, Skern T. Characterization and structure of the vaccinia virus NF-kappaB antagonist A46. J Biol Chem. 2014 Feb 7;289(6):3749-62. doi: 10.1074/jbc.M113.512756. Epub 2013, Dec 19. PMID:24356965 doi:http://dx.doi.org/10.1074/jbc.M113.512756
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