1jdm
From Proteopedia
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[[Image:1jdm.gif|left|200px]] | [[Image:1jdm.gif|left|200px]] | ||
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'''NMR Structure of Sarcolipin''' | '''NMR Structure of Sarcolipin''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JDM is a [[Single protein]] structure | + | 1JDM is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JDM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mascioni, A.]] | [[Category: Mascioni, A.]] | ||
[[Category: Veglia, G.]] | [[Category: Veglia, G.]] | ||
| - | [[Category: | + | [[Category: Helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:05:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:05, 2 May 2008
NMR Structure of Sarcolipin
Overview
Sarcolipin (SLN) is a 31 amino acid integral membrane protein that regulates Ca-ATPase activity in skeletal muscle. Here, we report the three-dimensional structure and topology of synthetic SLN in lipid environments, as determined by solution and solid-state NMR spectroscopy. 2D solution NMR experiments were performed on SLN solubilized in sodium dodecyl sulfate (SDS) micelles. We found that SLN adopts a highly defined alpha-helical conformation from F9 through R27, with a backbone RMSD of 0.65 A and a side chain RMSD of 1.66 A. The N-terminus (M1 through L8) and the C-terminus (S28 through Y31) are mostly unstructured. The orientation of the SLN was determined using one-dimensional (15)N NMR solid-state spectroscopy. The protein was incorporated into phospholipid bilayers prepared from a mixture of 1,2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. The (15)N chemical shift solid-state spectra from selectively labeled SLN samples indicate that SLN orients perpendicularly to the plane of the membrane bilayers. These results support the proposed mechanism of Ca-ATPase regulation of SLN via protein-protein intramembranous interactions between the highly conserved transmembrane domains of the Ca-ATPase and the conserved transmembrane domain of SLN.
About this Structure
1JDM is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structure and orientation of sarcolipin in lipid environments., Mascioni A, Karim C, Barany G, Thomas DD, Veglia G, Biochemistry. 2002 Jan 15;41(2):475-82. PMID:11781085 Page seeded by OCA on Fri May 2 21:05:49 2008
