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1jef
From Proteopedia
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[[Image:1jef.gif|left|200px]] | [[Image:1jef.gif|left|200px]] | ||
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'''TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3''' | '''TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3''' | ||
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[[Category: Harata, K.]] | [[Category: Harata, K.]] | ||
[[Category: Muraki, M.]] | [[Category: Muraki, M.]] | ||
| - | [[Category: | + | [[Category: Bacteriolytic enzyme]] |
| - | [[Category: | + | [[Category: Enzyme]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Inhibitor complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:07:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:07, 2 May 2008
TURKEY LYSOZYME COMPLEX WITH (GLCNAC)3
Overview
The turkey-egg lysozyme (TEL) complex with tri-N-acetylchitotriose [(GlcNac)3] was co-crystallized from 1.5% TEL and 2 mM (GlcNac)3 at pH 4.2. The crystal structure was determined by molecular replacement and refined to an R value of 0.182 using 10-1.77 A data. The (GlcNac)3 molecule occupies the subsites A, B and C. At the subsites B and C, the sugar residues are bound in a similar manner to that found in the hen-egg lysozyme (HEL) complex. In contrast, the GlcNac residue at the subsite A is exposed to bulk solvent and has no contact with the protein molecule because the active residue Asp101 in HEL is replaced by Gly in TEL. A sulfate ion is bound in the vicinity of subsite B and forms hydrogen bonds with the sugar residue and the guanidino group of Arg61, assisting the binding of the sugar residue to subsite B. The active-site cleft of TEL is narrower than that of native TEL, thus attaining the best fit of the (GlcNac)3 molecule. The lack of binding ability of subsite A is discussed in relation to the catalytic properties of TEL. The result suggests that the cleavage pattern of oligosaccharide substrates in the catalytic reaction is regulated by the protein-sugar interaction at subsite A.
About this Structure
1JEF is a Single protein structure of sequence from Meleagris gallopavo. Full crystallographic information is available from OCA.
Reference
X-ray structure of turkey-egg lysozyme complex with tri-N-acetylchitotriose. Lack of binding ability at subsite A., Harata K, Muraki M, Acta Crystallogr D Biol Crystallogr. 1997 Nov 1;53(Pt 6):650-7. PMID:15299852 Page seeded by OCA on Fri May 2 21:07:22 2008
