|
|
| Line 3: |
Line 3: |
| | <StructureSection load='1lr0' size='340' side='right'caption='[[1lr0]], [[Resolution|resolution]] 1.91Å' scene=''> | | <StructureSection load='1lr0' size='340' side='right'caption='[[1lr0]], [[Resolution|resolution]] 1.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1lr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LR0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1LR0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1lr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LR0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lr0 OCA], [https://pdbe.org/1lr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1lr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lr0 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TOLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1lr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lr0 OCA], [http://pdbe.org/1lr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1lr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lr0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOLA_PSEAE TOLA_PSEAE]] Involved in the TonB-independent uptake of proteins (By similarity). | + | [https://www.uniprot.org/uniprot/TOLA_PSEAE TOLA_PSEAE] Involved in the TonB-independent uptake of proteins (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 38: |
Line 36: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Grossman, J G]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Luisi, B]] | + | [[Category: Grossman JG]] |
| - | [[Category: Mizuguchi, K]] | + | [[Category: Luisi B]] |
| - | [[Category: Perham, R N]] | + | [[Category: Mizuguchi K]] |
| - | [[Category: Sanz, C]] | + | [[Category: Perham RN]] |
| - | [[Category: Shah, A]] | + | [[Category: Sanz C]] |
| - | [[Category: Witty, M]] | + | [[Category: Shah A]] |
| - | [[Category: Domain-swapping]]
| + | [[Category: Witty M]] |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Tola]]
| + | |
| - | [[Category: Tonb]]
| + | |
| Structural highlights
Function
TOLA_PSEAE Involved in the TonB-independent uptake of proteins (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of the C-terminal domain III of Pseudomonas aeruginosa TolA has been determined at 1.9 A resolution. The fold is similar to that of the corresponding domain of Escherichia coli TolA, despite the limited amino acid sequence identity of the two proteins (20%). A pattern was discerned that conserves the fold of domain III within the wider TolA family and, moreover, reveals a relationship between TolA domain III and the C-terminal domain of the TonB transporter proteins. We propose that the TolA and TonB C-terminal domains have a common evolutionary origin and are related by means of domain swapping, with interesting mechanistic implications. We have also determined the overall shape of the didomain, domains II + III, of P.aeruginosa TolA by solution X-ray scattering. The molecule is monomeric-its elongated, stalk shape can accommodate the crystal structure of domain III at one end, and an elongated helical bundle within the portion corresponding to domain II. Based on these data, a model for the periplasmic domains of P.aeruginosa TolA is presented that may explain the inferred allosteric properties of members of the TolA family. The mechanisms of TolA-mediated entry of bateriophages in P.aeruginosa and E.coli are likely to be similar.
Structure of the periplasmic domain of Pseudomonas aeruginosa TolA: evidence for an evolutionary relationship with the TonB transporter protein.,Witty M, Sanz C, Shah A, Grossmann JG, Mizuguchi K, Perham RN, Luisi B EMBO J. 2002 Aug 15;21(16):4207-18. PMID:12169623[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Witty M, Sanz C, Shah A, Grossmann JG, Mizuguchi K, Perham RN, Luisi B. Structure of the periplasmic domain of Pseudomonas aeruginosa TolA: evidence for an evolutionary relationship with the TonB transporter protein. EMBO J. 2002 Aug 15;21(16):4207-18. PMID:12169623
|
