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1t0k

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Revision as of 09:53, 21 December 2022

Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex

Structural highlights

1t0k is a 4 chain structure with sequence from Escherichia coli and Saccharomyces cerevisiae. This structure supersedes the now removed PDB entries 1ck5, 1ck8, 1cn8 and 1cn9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL30_YEAST

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

L30e, a Saccharomyces cervisiae ribosomal protein, regulates its own expression by binding to a purine-rich asymmetric internal loop located in both its pre-mRNA and mature mRNA. A crystal structure of an MBP-L30e fusion protein in complex with an RNA containing the pre-mRNA regulatory site was solved at 3.24 A. Interestingly, the structure of the RNA differed from that observed in a previously determined NMR structure of the complex. Analysis of the NMR data led to the identification of a single imino proton resonance in the internal loop that had been incorrectly assigned and was principally responsible for the erroneous RNA structure. A structure refinement was performed using both the X-ray diffraction data and the NMR-derived distance and angle restraints. The joint NMR and X-ray refinement resulted in improved stereochemistry and lower crystallographic R factors. The RNA internal loop of the MBP-L30e-mRNA complex adopts the canonical K-turn fold.

Joint X-ray and NMR refinement of the yeast L30e-mRNA complex.,Chao JA, Williamson JR Structure. 2004 Jul;12(7):1165-76. PMID:15242593^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao JA, Williamson JR. Joint X-ray and NMR refinement of the yeast L30e-mRNA complex. Structure. 2004 Jul;12(7):1165-76. PMID:15242593 doi:10.1016/j.str.2004.04.023

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t0k"

Categories: Escherichia coli | Large Structures | Saccharomyces cerevisiae | Chao JA | Williamson JR

@@ Line 3: / Line 3: @@
 <StructureSection load='1t0k' size='340' side='right'caption='[[1t0k]], [[Resolution|resolution]] 3.24&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1t0k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ck5 1ck5], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ck8 1ck8], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cn8 1cn8] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cn9 1cn9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T0K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1t0k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ck5 1ck5], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ck8 1ck8], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cn8 1cn8] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cn9 1cn9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0K FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MALE, B4034, Z5632, ECS5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), RPL30, RPL32, YGL030W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0k OCA], [https://pdbe.org/1t0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0k RCSB], [https://www.ebi.ac.uk/pdbsum/1t0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0k ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0k OCA], [http://pdbe.org/1t0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t0k RCSB], [http://www.ebi.ac.uk/pdbsum/1t0k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0k ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
+[https://www.uniprot.org/uniprot/RL30_YEAST RL30_YEAST]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 39: / Line 38: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Chao, J A]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Williamson, J R]]
+[[Category: Chao JA]]
-[[Category: Joint nmr and x-ray refinement]]
+[[Category: Williamson JR]]
-[[Category: Mbp fusion protein]]
-[[Category: Ribosomal protein l30e]]
-[[Category: Ribosome]]

1t0k

From Proteopedia

Revision as of 09:53, 21 December 2022

Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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