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| | <StructureSection load='6zj8' size='340' side='right'caption='[[6zj8]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='6zj8' size='340' side='right'caption='[[6zj8]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6zj8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Borpe Borpe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJ8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zj8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJ8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bvgS, BP1877 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=257313 BORPE])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj8 OCA], [https://pdbe.org/6zj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zj8 RCSB], [https://www.ebi.ac.uk/pdbsum/6zj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj8 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj8 OCA], [https://pdbe.org/6zj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zj8 RCSB], [https://www.ebi.ac.uk/pdbsum/6zj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/BVGS_BORPE BVGS_BORPE]] Member of the two-component regulatory system BvgS/BvgA. Phosphorylates BvgA via a four-step phosphorelay in response to environmental signals.
| + | [https://www.uniprot.org/uniprot/BVGS_BORPE BVGS_BORPE] Member of the two-component regulatory system BvgS/BvgA. Phosphorylates BvgA via a four-step phosphorelay in response to environmental signals. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Borpe]] | + | [[Category: Bordetella pertussis Tohama I]] |
| - | [[Category: Histidine kinase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Clantin, B]] | + | [[Category: Clantin B]] |
| - | [[Category: Dupre, E]] | + | [[Category: Dupre E]] |
| - | [[Category: Jacob-Dubuisson, F]] | + | [[Category: Jacob-Dubuisson F]] |
| - | [[Category: Villeret, V]] | + | [[Category: Villeret V]] |
| - | [[Category: Pas domain sensor histidine-kinase]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
BVGS_BORPE Member of the two-component regulatory system BvgS/BvgA. Phosphorylates BvgA via a four-step phosphorelay in response to environmental signals.
Publication Abstract from PubMed
The two-component system BvgAS controls the virulence regulon in Bordetella pertussis BvgS is the prototype of a family of sensor histidine-kinases harboring periplasmic Venus flytrap (VFT) domains. The VFT domains are connected to the cytoplasmic kinase moiety by helical linkers separated by a Per-ARNT-Sim (PAS) domain. Antagonism between the two linkers, as one forms a coiled coil when the other is dynamic and vice versa, regulates BvgS activity. Here we solved the structure of the intervening PAS domain by X-ray crystallography. Two forms were obtained that notably differ by the connections between the PAS core domain and the flanking helical linkers. Structure-guided mutagenesis indicated that those connections participate in the regulation of BvgS activity. The PAS domain thus appears to function as a switch-facilitator module whose conformation determines the output of the system. As many BvgS homologs have similar architectures, the mechanisms unveiled here are likely to generally apply to the regulation of sensor-histidine kinases of that family.IMPORTANCEThe whooping cough agent Bordetella pertussis colonizes the human respiratory tract using virulence factors co-regulated by the sensory transduction system BvgAS. BvgS is a model for a family of sensor-kinase proteins, some of which are found in important bacterial pathogens. BvgS functions as a kinase or a phosphatase depending on external signals, which determines if B. pertussis is virulent or avirulent. Deciphering its mode of action might thus lead to new ways of fighting infections. Here we used X-ray crystallography to solve the three-dimensional structure of the domain that precedes the enzymatic moiety and identified features that regulate BvgS activity. As many sensor-kinases of the BvgS family harbor homologous domains, the mechanism unveiled here might be of general relevance.
Structural insight into the role of the PAS domainfor signal transduction in sensor-kinase BvgS.,Dupre E, Clantin B, Yuan Y, Lecher S, Lesne E, Antoine R, Villeret V, Jacob-Dubuisson F J Bacteriol. 2021 Feb 22. pii: JB.00614-20. doi: 10.1128/JB.00614-20. PMID:33619154[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dupre E, Clantin B, Yuan Y, Lecher S, Lesne E, Antoine R, Villeret V, Jacob-Dubuisson F. Structural insight into the role of the PAS domainfor signal transduction in sensor-kinase BvgS. J Bacteriol. 2021 Feb 22. pii: JB.00614-20. doi: 10.1128/JB.00614-20. PMID:33619154 doi:http://dx.doi.org/10.1128/JB.00614-20
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