1jf8
From Proteopedia
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'''X-ray structure of reduced C10S, C15A arsenate reductase from pI258''' | '''X-ray structure of reduced C10S, C15A arsenate reductase from pI258''' | ||
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[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: Zegers, I.]] | [[Category: Zegers, I.]] | ||
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| - | [[Category: | + | [[Category: Ptpase i fold]] |
| - | [[Category: | + | [[Category: Sulfinic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:09:10 2008'' | |
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Revision as of 18:09, 2 May 2008
X-ray structure of reduced C10S, C15A arsenate reductase from pI258
Overview
Arsenate reductase (ArsC) from Staphylococcus aureus plasmid pI258 plays a role in bacterial heavy metal resistance and catalyzes the reduction of arsenate to arsenite. The structures of the oxidized and reduced forms of ArsC were solved. ArsC has the PTPase I fold typical for low molecular weight tyrosine phosphatases (LMW PTPases). Remarkably, kinetic experiments show that pI258 ArsC also catalyzes the tyrosine phosphatase reaction in addition to arsenate reduction. These results provide evidence that ArsC from pI258 evolved from LMW PTPase by the grafting of a redox function onto a pre-existing catalytic site and that its evolutionary origin is different from those of arsenate reductases from Escherichia coli plasmid R773 and from Saccharomyces cerevisiae. The mechanism proposed here for the catalysis of arsenate reduction by pI258 ArsC involves a nucleophilic attack by Cys 10 on arsenate, the formation of a covalent intermediate and the transport of oxidative equivalents by a disulfide cascade. The reaction is associated with major structural changes in the ArsC.
About this Structure
1JF8 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Arsenate reductase from S. aureus plasmid pI258 is a phosphatase drafted for redox duty., Zegers I, Martins JC, Willem R, Wyns L, Messens J, Nat Struct Biol. 2001 Oct;8(10):843-7. PMID:11573087 Page seeded by OCA on Fri May 2 21:09:10 2008
