7p2p
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Human Signal Peptidase Complex Paralog A (SPC-A)== |
| - | <StructureSection load='7p2p' size='340' side='right'caption='[[7p2p]]' scene=''> | + | <StructureSection load='7p2p' size='340' side='right'caption='[[7p2p]], [[Resolution|resolution]] 4.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p2p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P2P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P2P FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p2p OCA], [https://pdbe.org/7p2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p2p RCSB], [https://www.ebi.ac.uk/pdbsum/7p2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p2p ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p2p OCA], [https://pdbe.org/7p2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p2p RCSB], [https://www.ebi.ac.uk/pdbsum/7p2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p2p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SC11A_HUMAN SC11A_HUMAN] Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed:34388369). Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids (PubMed:34388369).<ref>PMID:34388369</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The signal peptidase complex (SPC) is an essential membrane complex in the endoplasmic reticulum (ER), where it removes signal peptides (SPs) from a large variety of secretory pre-proteins with exquisite specificity. Although the determinants of this process have been established empirically, the molecular details of SP recognition and removal remain elusive. Here, we show that the human SPC exists in two functional paralogs with distinct proteolytic subunits. We determined the atomic structures of both paralogs using electron cryo-microscopy and structural proteomics. The active site is formed by a catalytic triad and abuts the ER membrane, where a transmembrane window collectively formed by all subunits locally thins the bilayer. Molecular dynamics simulations indicate that this unique architecture generates specificity for SPs based on the length of their hydrophobic segments. | ||
| + | |||
| + | Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.,Liaci AM, Steigenberger B, Telles de Souza PC, Tamara S, Grollers-Mulderij M, Ogrissek P, Marrink SJ, Scheltema RA, Forster F Mol Cell. 2021 Oct 7;81(19):3934-3948.e11. doi: 10.1016/j.molcel.2021.07.031. , Epub 2021 Aug 12. PMID:34388369<ref>PMID:34388369</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p2p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Foerster F]] |
| + | [[Category: Liaci AM]] | ||
Current revision
Human Signal Peptidase Complex Paralog A (SPC-A)
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