1ev7

From Proteopedia

(Difference between revisions)

Revision as of 10:25, 21 December 2022

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI

Structural highlights

1ev7 is a 2 chain structure with sequence from Lentzea aerocolonigenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2N1_LENAE An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.^[1] ^[2] ^[3]

Publication Abstract from PubMed

NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes.

Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.,Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jo K, Topal MD. DNA topoisomerase and recombinase activities in Nae I restriction endonuclease. Science. 1995 Mar 24;267(5205):1817-20. doi: 10.1126/science.7892605. PMID:7892605 doi:http://dx.doi.org/10.1126/science.7892605
↑ Jo K, Topal MD. Effects on NaeI-DNA recognition of the leucine to lysine substitution that transforms restriction endonuclease NaeI to a topoisomerase: a model for restriction endonuclease evolution. Nucleic Acids Res. 1996 Nov 1;24(21):4171-5. doi: 10.1093/nar/24.21.4171. PMID:8932368 doi:http://dx.doi.org/10.1093/nar/24.21.4171
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kruger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1;31(7):1805-12. PMID:12654995
↑ Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H. Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase. EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254 doi:10.1093/emboj/19.12.3110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ev7"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ev7' size='340' side='right'caption='[[1ev7]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/T2N1_NOCAE T2N1_NOCAE]] Recognizes the double-stranded unmethylated sequence GCCGGC and cleaves after C-3.
+[https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 25: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Type II site-specific deoxyribonuclease]]
+[[Category: Lentzea aerocolonigenes]]
-[[Category: Chen, Y]]
+[[Category: Chen Y]]
-[[Category: Colandene, J D]]
+[[Category: Colandene JD]]
-[[Category: Huai, Q]]
+[[Category: Huai Q]]
-[[Category: Luo, F]]
+[[Category: Luo F]]
-[[Category: Zhao, Y]]
+[[Category: Zhao Y]]
-[[Category: Apo-naei]]
-[[Category: Cap]]
-[[Category: Helix-turn-helix]]
-[[Category: Hydrolase]]
-[[Category: Restriction endonuclease]]
-[[Category: Topoisomerase]]

1ev7

From Proteopedia

Revision as of 10:25, 21 December 2022

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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