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4lvi

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Revision as of 10:35, 21 December 2022

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 4.6

Structural highlights

4lvi is a 3 chain structure with sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRE_STRAG The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.

Publication Abstract from PubMed

Relaxases are metal-dependent nucleases that break and join DNA for the initiation and completion of conjugative bacterial gene transfer. Conjugation is the main process through which antibiotic resistance spreads among bacteria, with multidrug-resistant staphylococci and streptococci infections posing major threats to human health. The MOBV family of relaxases accounts for approximately 85% of all relaxases found in Staphylococcus aureus isolates. Here, we present six structures of the MOBV relaxase MobM from the promiscuous plasmid pMV158 in complex with several origin of transfer DNA fragments. A combined structural, biochemical, and computational approach reveals that MobM follows a previously uncharacterized histidine/metal-dependent DNA processing mechanism, which involves the formation of a covalent phosphoramidate histidine-DNA adduct for cell-to-cell transfer. We discuss how the chemical features of the high-energy phosphorus-nitrogen bond shape the dominant position of MOBV histidine relaxases among small promiscuous plasmids and their preference toward Gram-positive bacteria.

Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.,Pluta R, Boer DR, Lorenzo-Diaz F, Russi S, Gomez H, Fernandez-Lopez C, Perez-Luque R, Orozco M, Espinosa M, Coll M Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6526-E6535. doi:, 10.1073/pnas.1702971114. Epub 2017 Jul 24. PMID:28739894^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pluta R, Boer DR, Lorenzo-Diaz F, Russi S, Gomez H, Fernandez-Lopez C, Perez-Luque R, Orozco M, Espinosa M, Coll M. Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6526-E6535. doi:, 10.1073/pnas.1702971114. Epub 2017 Jul 24. PMID:28739894 doi:http://dx.doi.org/10.1073/pnas.1702971114

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lvi"

Categories: Large Structures | Streptococcus agalactiae | Boer DR | Coll M | Pluta R

@@ Line 3: / Line 3: @@
 <StructureSection load='4lvi' size='340' side='right'caption='[[4lvi]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4lvi]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LVI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4lvi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LVI FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lvj|4lvj]], [[4lvk|4lvk]], [[4lvl|4lvl]], [[4lvm|4lvm]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvi OCA], [https://pdbe.org/4lvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lvi RCSB], [https://www.ebi.ac.uk/pdbsum/4lvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvi ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvi OCA], [http://pdbe.org/4lvi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lvi RCSB], [http://www.ebi.ac.uk/pdbsum/4lvi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvi ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG]] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.
+[https://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Boer, D R]]
+[[Category: Streptococcus agalactiae]]
-[[Category: Coll, M]]
+[[Category: Boer DR]]
-[[Category: Pluta, R]]
+[[Category: Coll M]]
-[[Category: Dna binding protein-dna complex]]
+[[Category: Pluta R]]
-[[Category: Orit dna]]
-[[Category: Protein-dna complex]]
-[[Category: Relaxase/endonuclease]]

4lvi

From Proteopedia

Revision as of 10:35, 21 December 2022

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 4.6

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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