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| | ==Crystal structure of IFT81 N-terminal domain== | | ==Crystal structure of IFT81 N-terminal domain== |
| - | <StructureSection load='4lvp' size='340' side='right' caption='[[4lvp]], [[Resolution|resolution]] 2.32Å' scene=''> | + | <StructureSection load='4lvp' size='340' side='right'caption='[[4lvp]], [[Resolution|resolution]] 2.32Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lvp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LVP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lvp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LVP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TA0:TANTALUM'>TA0</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TA0:TANTALUM'>TA0</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lvr|4lvr]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvp OCA], [https://pdbe.org/4lvp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lvp RCSB], [https://www.ebi.ac.uk/pdbsum/4lvp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvp ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IFT81, CHLREDRAFT_138649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvp OCA], [http://pdbe.org/4lvp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lvp RCSB], [http://www.ebi.ac.uk/pdbsum/4lvp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/IFT81_CHLRE IFT81_CHLRE] Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis.<ref>PMID:23990561</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chlre]] | + | [[Category: Chlamydomonas reinhardtii]] |
| - | [[Category: Bhogaraju, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Ch domain]] | + | [[Category: Bhogaraju S]] |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Tubulin/microtubule]]
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| Structural highlights
Function
IFT81_CHLRE Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis.[1]
Publication Abstract from PubMed
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.,Bhogaraju S, Cajanek L, Fort C, Blisnick T, Weber K, Taschner M, Mizuno N, Lamla S, Bastin P, Nigg EA, Lorentzen E Science. 2013 Aug 30;341(6149):1009-12. doi: 10.1126/science.1240985. PMID:23990561[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bhogaraju S, Cajanek L, Fort C, Blisnick T, Weber K, Taschner M, Mizuno N, Lamla S, Bastin P, Nigg EA, Lorentzen E. Molecular basis of tubulin transport within the cilium by IFT74 and IFT81. Science. 2013 Aug 30;341(6149):1009-12. doi: 10.1126/science.1240985. PMID:23990561 doi:10.1126/science.1240985
- ↑ Bhogaraju S, Cajanek L, Fort C, Blisnick T, Weber K, Taschner M, Mizuno N, Lamla S, Bastin P, Nigg EA, Lorentzen E. Molecular basis of tubulin transport within the cilium by IFT74 and IFT81. Science. 2013 Aug 30;341(6149):1009-12. doi: 10.1126/science.1240985. PMID:23990561 doi:10.1126/science.1240985
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