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| | ==Crystal Structure of MurQ from H.influenzae with bound inhibitor== | | ==Crystal Structure of MurQ from H.influenzae with bound inhibitor== |
| - | <StructureSection load='4lzj' size='340' side='right' caption='[[4lzj]], [[Resolution|resolution]] 2.41Å' scene=''> | + | <StructureSection load='4lzj' size='340' side='right'caption='[[4lzj]], [[Resolution|resolution]] 2.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lzj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LZJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lzj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LZJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=22H:2-(ACETYLAMINO)-3-O-[(1R)-1-CARBOXYETHYL]-2-DEOXY-6-O-PHOSPHONO-D-GLUCITOL'>22H</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=22H:2-(ACETYLAMINO)-3-O-[(1R)-1-CARBOXYETHYL]-2-DEOXY-6-O-PHOSPHONO-D-GLUCITOL'>22H</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m0d|4m0d]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lzj OCA], [https://pdbe.org/4lzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lzj RCSB], [https://www.ebi.ac.uk/pdbsum/4lzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lzj ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">murQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramic_acid_6-phosphate_etherase N-acetylmuramic acid 6-phosphate etherase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.126 4.2.1.126] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lzj OCA], [http://pdbe.org/4lzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lzj RCSB], [http://www.ebi.ac.uk/pdbsum/4lzj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lzj ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MURQ_HAEIN MURQ_HAEIN]] Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity). | + | [https://www.uniprot.org/uniprot/MURQ_HAEIN MURQ_HAEIN] Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium influenzae lehmann and neumann 1896]] | + | [[Category: Haemophilus influenzae]] |
| - | [[Category: N-acetylmuramic acid 6-phosphate etherase]] | + | [[Category: Large Structures]] |
| - | [[Category: Blanchard, J]] | + | [[Category: Blanchard J]] |
| - | [[Category: Hazra, S]] | + | [[Category: Hazra S]] |
| - | [[Category: Alpha-beta-alpha sandwich]]
| + | |
| - | [[Category: D-muramitol 6-phosphate]]
| + | |
| - | [[Category: Lyase-lyase inhibitor complex]]
| + | |
| - | [[Category: Murq]]
| + | |
| - | [[Category: Protein-ligand complex]]
| + | |
| - | [[Category: Yfeu]]
| + | |
| Structural highlights
Function
MURQ_HAEIN Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity).
Publication Abstract from PubMed
The breakdown and recycling of peptidoglycan, an essential polymeric cell structure, occur in a number of bacterial species. A key enzyme in the recycling pathway of one of the components of the peptidoglycan layer, N-acetylmuramic acid (MurNAc), is MurNAc 6-phosphate hydrolase (MurQ). This enzyme catalyzes the cofactor-independent cleavage of a relatively nonlabile ether bond and presents an interesting target for mechanistic studies. Open chain product and substrate analogues were synthesized and tested as competitive inhibitors (Kis values of 1.1 +/- 0.3 and 0.23 +/- 0.02 mM, respectively) of the MurNAc 6P hydrolase from Escherichia coli (MurQ-EC). To identify the roles of active site residues that are important for catalysis, the substrate analogue was cocrystallized with the MurNAc 6P hydrolase from Haemophilus influenzae (MurQ-HI) that was amenable to crystallographic studies. The cocrystal structure of MurQ-HI with the substrate analogue showed that Glu89 was located in the proximity of both the C2 atom and the oxygen at the C3 position of the bound inhibitor and that no other potential acid/base residue that could act as an active site acid/base was located in the vicinity. The conserved residues Glu120 and Lys239 were found within hydrogen bonding distance of the C5 hydroxyl group and C6 phosphate group, suggesting that they play a role in substrate binding and ring opening. Combining these results with previous biochemical data, we propose a one-base mechanism of action in which Glu89 functions to both deprotonate at the C2 position and assist in the departure of the lactyl ether at the C3 position. This same residue would serve to deprotonate the incoming water and reprotonate the enolate in the second half of the catalytic cycle.
Structure of MurNAc 6-phosphate hydrolase (MurQ) from Haemophilus influenzae with a bound inhibitor.,Hadi T, Hazra S, Tanner ME, Blanchard JS Biochemistry. 2013 Dec 23;52(51):9358-66. doi: 10.1021/bi4010446. Epub 2013 Nov, 22. PMID:24251551[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hadi T, Hazra S, Tanner ME, Blanchard JS. Structure of MurNAc 6-phosphate hydrolase (MurQ) from Haemophilus influenzae with a bound inhibitor. Biochemistry. 2013 Dec 23;52(51):9358-66. doi: 10.1021/bi4010446. Epub 2013 Nov, 22. PMID:24251551 doi:http://dx.doi.org/10.1021/bi4010446
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