|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of hypothetical protein SAV0944 from Staphylococcus aureus subsp. aureus Mu50== | | ==Crystal Structure of hypothetical protein SAV0944 from Staphylococcus aureus subsp. aureus Mu50== |
| - | <StructureSection load='4m20' size='340' side='right' caption='[[4m20]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4m20' size='340' side='right'caption='[[4m20]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4m20]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M20 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4m20]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M20 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV0944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m20 OCA], [https://pdbe.org/4m20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m20 RCSB], [https://www.ebi.ac.uk/pdbsum/4m20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m20 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m20 OCA], [http://pdbe.org/4m20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m20 RCSB], [http://www.ebi.ac.uk/pdbsum/4m20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m20 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3JUJ1_STAAM A0A0H3JUJ1_STAAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staam]] | + | [[Category: Large Structures]] |
| - | [[Category: Forwood, J K]] | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| - | [[Category: Khandokar, Y B]] | + | [[Category: Forwood JK]] |
| - | [[Category: Hot dog fold]] | + | [[Category: Khandokar YB]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Hydrolysis of thioester bond]]
| + | |
| - | [[Category: Thioesterase]]
| + | |
| Structural highlights
Function
A0A0H3JUJ1_STAAM
Publication Abstract from PubMed
Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 A resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 A, beta = 100.5 degrees . Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement.
Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.,Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):244-7. doi:, 10.1107/S2053230X14000338. Epub 2014 Jan 22. PMID:24637766[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK. Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):244-7. doi:, 10.1107/S2053230X14000338. Epub 2014 Jan 22. PMID:24637766 doi:http://dx.doi.org/10.1107/S2053230X14000338
|
