4m4s

From Proteopedia

(Difference between revisions)

Revision as of 10:55, 21 December 2022

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gammaGDPPi complex (a formate ion substitutes for Pi)

Structural highlights

4m4s is a 1 chain structure with sequence from Saccharolobus solfataricus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF2G_SACS2 eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.[HAMAP-Rule:MF_00119]

Publication Abstract from PubMed

In eukaryotes and archaea, the heterotrimeric translation initiation factor 2 (e/aIF2) is pivotal for the delivery of methionylated initiator tRNA (Met-tRNA(i)) to the ribosome. It acts as a molecular switch that cycles between inactive (GDP-bound) and active (GTP-bound) states. Recent studies show that eIF2 can also exist in a long-lived eIF2gamma-GDP-P(i) (inorganic phosphate) active state. Here, four high-resolution crystal structures of aIF2gamma from Sulfolobus solfataricus are reported: aIF2gamma-GDPCP (a nonhydrolyzable GTP analogue), aIF2gamma-GDP-formate (in which a formate ion possibly mimics P(i)), aIF2gamma-GDP and nucleotide-free aIF2gamma. The structures describe the different states of aIF2gamma and demonstrate the conformational transitions that take place in the aIF2gamma `life cycle'.

Conformational transitions in the gamma subunit of the archaeal translation initiation factor 2.,Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):658-67. doi:, 10.1107/S1399004713032240. Epub 2014 Feb 15. PMID:24598735^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M. Conformational transitions in the gamma subunit of the archaeal translation initiation factor 2. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):658-67. doi:, 10.1107/S1399004713032240. Epub 2014 Feb 15. PMID:24598735 doi:http://dx.doi.org/10.1107/S1399004713032240

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4m4s"

@@ Line 3: / Line 3: @@
 <StructureSection load='4m4s' size='340' side='right'caption='[[4m4s]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4m4s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M4S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M4S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4m4s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M4S FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m0l|4m0l]], [[4m2l|4m2l]], [[4m53|4m53]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m4s OCA], [https://pdbe.org/4m4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m4s RCSB], [https://www.ebi.ac.uk/pdbsum/4m4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m4s ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eif2g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m4s OCA], [http://pdbe.org/4m4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m4s RCSB], [http://www.ebi.ac.uk/pdbsum/4m4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m4s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IF2G_SULSO IF2G_SULSO]] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).
+[https://www.uniprot.org/uniprot/IF2G_SACS2 IF2G_SACS2] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.[HAMAP-Rule:MF_00119]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Sacs2]]
+[[Category: Saccharolobus solfataricus P2]]
-[[Category: Arkhipova, V I]]
+[[Category: Arkhipova VI]]
-[[Category: Gabdulkhakov, A G]]
+[[Category: Gabdulkhakov AG]]
-[[Category: Garber, M B]]
+[[Category: Garber MB]]
-[[Category: Nikonov, O S]]
+[[Category: Nikonov OS]]
-[[Category: Nikonov, S V]]
+[[Category: Nikonov SV]]
-[[Category: Nikulin, A D]]
+[[Category: Nikulin AD]]
-[[Category: Stolboushkina, E A]]
+[[Category: Stolboushkina EA]]
-[[Category: Aif2]]
-[[Category: Aif2 gamma subunit]]
-[[Category: G-protein]]
-[[Category: Gtp-binding]]
-[[Category: Nucleotide-binding]]
-[[Category: Protein biosynthesis]]
-[[Category: Rna-binding]]
-[[Category: Translation]]
-[[Category: Translation initiation]]

4m4s

From Proteopedia

Revision as of 10:55, 21 December 2022

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gammaGDPPi complex (a formate ion substitutes for Pi)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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Views

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4m4s

From Proteopedia

Revision as of 10:55, 21 December 2022

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gamma*GDP*Pi complex (a formate ion substitutes for Pi)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gammaGDPPi complex (a formate ion substitutes for Pi)