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| | ==Crystal structure of the Utp22 and Rrp7 complex from Saccharomyces cerevisiae== | | ==Crystal structure of the Utp22 and Rrp7 complex from Saccharomyces cerevisiae== |
| - | <StructureSection load='4m5d' size='340' side='right' caption='[[4m5d]], [[Resolution|resolution]] 1.97Å' scene=''> | + | <StructureSection load='4m5d' size='340' side='right'caption='[[4m5d]], [[Resolution|resolution]] 1.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4m5d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M5D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4m5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M5D FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UTP22, YGR090W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), RRP7, YCL031C, YCL184, YCL31C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m5d OCA], [https://pdbe.org/4m5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m5d RCSB], [https://www.ebi.ac.uk/pdbsum/4m5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m5d ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m5d OCA], [http://pdbe.org/4m5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m5d RCSB], [http://www.ebi.ac.uk/pdbsum/4m5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m5d ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UTP22_YEAST UTP22_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref> [[http://www.uniprot.org/uniprot/RRP7_YEAST RRP7_YEAST]] Plays an important role in the synthesis of 18S rRNA but is not required for the 5.8S and 25S pathway. Is necessary for the cleavage at site A2. Is required for efficient association of RPS27 with the pre-ribosomal particle. | + | [https://www.uniprot.org/uniprot/UTP22_YEAST UTP22_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Lin, J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ye, K]] | + | [[Category: Lin J]] |
| - | [[Category: Nucleolus]] | + | [[Category: Ye K]] |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
UTP22_YEAST Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[1]
Publication Abstract from PubMed
A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain, are components of the 90S preribosome or the small subunit processome that conducts early processing of 18S rRNA. Here, we determine the cocrystal structure of Utp22 and Rrp7 complex at 1.97 A resolution and the NMR structure of a C-terminal fragment of Rrp7, which is not visible in the crystal structure. The structure reveals that Utp22 surprisingly resembles a dimeric class I tRNA CCA-adding enzyme yet with degenerate active sites, raising an interesting evolutionary connection between tRNA and rRNA processing machineries. Rrp7 binds extensively to Utp22 using a deviant RNA recognition motif and an extended linker. Functional sites on the two proteins were identified by structure-based mutagenesis in yeast. We show that Rrp7 contains a flexible RNA-binding C-terminal tail that is essential for association with preribosomes. RNA-protein crosslinking shows that Rrp7 binds at the central domain of 18S rRNA and shares a neighborhood with two processing H/ACA snoRNAs snR30 and snR10. Depletion of snR30 prevents the stable assembly of Rrp7 into preribosomes. Our results provide insight into the evolutionary origin and functional context of Utp22 and Rrp7.
An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme.,Lin J, Lu J, Feng Y, Sun M, Ye K PLoS Biol. 2013 Oct;11(10):e1001669. doi: 10.1371/journal.pbio.1001669. Epub 2013, Oct 1. PMID:24130456[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ. The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell. 2004 Dec;3(6):1619-26. PMID:15590835 doi:http://dx.doi.org/10.1128/EC.3.6.1619-1626.2004
- ↑ Lin J, Lu J, Feng Y, Sun M, Ye K. An RNA-Binding Complex Involved in Ribosome Biogenesis Contains a Protein with Homology to tRNA CCA-Adding Enzyme. PLoS Biol. 2013 Oct;11(10):e1001669. doi: 10.1371/journal.pbio.1001669. Epub 2013, Oct 1. PMID:24130456 doi:http://dx.doi.org/10.1371/journal.pbio.1001669
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