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4m70

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Revision as of 11:00, 21 December 2022

Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain

Structural highlights

4m70 is a 10 chain structure with sequence from Solanum tuberosum and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9XGF5_SOLTU

Publication Abstract from PubMed

The potato (Solanum tuberosum) disease resistance protein Rx has a modular arrangement that contains coiled-coil (CC), nucleotide-binding (NB), and leucine-rich repeat (LRR) domains and mediates resistance to potato virus X. The Rx N-terminal CC domain undergoes an intramolecular interaction with the Rx NB-LRR region and an intermolecular interaction with the Rx cofactor RanGAP2 (Ran GTPase-activating protein 2). Here, we report the crystal structure of the Rx CC domain in complex with the Trp-Pro-Pro (WPP) domain of RanGAP2. The structure reveals that the Rx CC domain forms a heterodimer with RanGAP2, in striking contrast to the homodimeric structure of the CC domain of the barley disease resistance protein MLA10. Structure-based mutagenesis identified residues from both the Rx CC domain and the RanGAP2 WPP domain that are crucial for their interaction and function in vitro and in vivo. Our results reveal the molecular mechanism underlying the interaction of Rx with RanGAP2 and identify the distinct surfaces of the Rx CC domain that are involved in intramolecular and intermolecular interactions.

Structural Basis for the Interaction between the Potato Virus X Resistance Protein (Rx) and Its Cofactor Ran GTPase-activating Protein 2 (RanGAP2).,Hao W, Collier SM, Moffett P, Chai J J Biol Chem. 2013 Dec 13;288(50):35868-76. doi: 10.1074/jbc.M113.517417. Epub, 2013 Nov 5. PMID:24194517^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hao W, Collier SM, Moffett P, Chai J. Structural Basis for the Interaction between the Potato Virus X Resistance Protein (Rx) and Its Cofactor Ran GTPase-activating Protein 2 (RanGAP2). J Biol Chem. 2013 Dec 13;288(50):35868-76. doi: 10.1074/jbc.M113.517417. Epub, 2013 Nov 5. PMID:24194517 doi:http://dx.doi.org/10.1074/jbc.M113.517417

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4m70"

Categories: Large Structures | Solanum tuberosum | Synthetic construct | Chai J | Hao W

@@ Line 3: / Line 3: @@
 <StructureSection load='4m70' size='340' side='right'caption='[[4m70]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4m70]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M70 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4m70]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M70 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m70 OCA], [https://pdbe.org/4m70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m70 RCSB], [https://www.ebi.ac.uk/pdbsum/4m70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m70 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m70 OCA], [http://pdbe.org/4m70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m70 RCSB], [http://www.ebi.ac.uk/pdbsum/4m70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m70 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9XGF5_SOLTU Q9XGF5_SOLTU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Chai, J]]
+[[Category: Solanum tuberosum]]
-[[Category: Hao, W]]
+[[Category: Synthetic construct]]
-[[Category: Coiled coil domain]]
+[[Category: Chai J]]
-[[Category: Plant disease resistance gene]]
+[[Category: Hao W]]
-[[Category: Plant protein]]
-[[Category: Popato x virus]]
-[[Category: Rangap2]]
-[[Category: Resistance response]]
-[[Category: Rx]]
-[[Category: Wpp domain]]

4m70

From Proteopedia

Revision as of 11:00, 21 December 2022

Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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