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| | ==Crystal structure of calcium-bound annexin (Sm)1== | | ==Crystal structure of calcium-bound annexin (Sm)1== |
| - | <StructureSection load='4mdv' size='340' side='right' caption='[[4mdv]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4mdv' size='340' side='right'caption='[[4mdv]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mdv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MDV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mdv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MDV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mdu|4mdu]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdv OCA], [https://pdbe.org/4mdv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mdv RCSB], [https://www.ebi.ac.uk/pdbsum/4mdv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdv ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">annexin (Sm)1, Smp_074150.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdv OCA], [http://pdbe.org/4mdv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mdv RCSB], [http://www.ebi.ac.uk/pdbsum/4mdv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C4QH88_SCHMA C4QH88_SCHMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Annexin|Annexin]] | + | *[[Annexin 3D structures|Annexin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blood fluke]] | + | [[Category: Large Structures]] |
| - | [[Category: Hofmann, A]] | + | [[Category: Schistosoma mansoni]] |
| - | [[Category: Annexin]] | + | [[Category: Hofmann A]] |
| - | [[Category: Calcium-binding protein]]
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| - | [[Category: Metal binding protein]]
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| Structural highlights
Function
C4QH88_SCHMA
Publication Abstract from PubMed
Schistosomiasis is a major parasitic disease of humans, second only to malaria in its global impact. The disease is caused by digenean trematodes that infest the vasculature of their human hosts. These flukes are limited externally by a body wall composed of a syncytial epithelium, the apical surface membrane of which is a parasitism-adapted dual membrane complex. Annexins are thought to be of integral importance for the stability of this apical membrane system. Here, we present the first structural and immunobiochemical characterization of an annexin from Schistosoma mansoni. The crystal structure of annexin B22 confirms the presence of the previously predicted alpha-helical segment in the II/III linker and reveals a covalently linked head-to-head dimer. From the calcium-bound crystal structure of this protein, canonical type II, type III and B site positions are occupied, and a novel binding site has been identified. The dimer arrangement observed in the crystal structure suggests the presence of two prominent features, a potential non-canonical membrane binding site and a potential binding groove opposite to the former. Results from transcriptional profiling during development show that annexin B22 expression is correlated with life stages of the parasite that possess the syncytial tegument layer, and ultrastructural localization by immuno-electron microscopy confirms the occurrence of annexins in the tegument of S. mansoni. Data from membrane binding and aggregation assays indicate the presence of differential molecular mechanisms and support the hypothesis of annexin B22 providing structural integrity in the tegument. DATABASE: Coordinates and structure factors have been deposited with the PDB, accession numbers 4mdu, 4mdv STRUCTURED DIGITAL ABSTRACT: annexin B22 and annexin B22 bind by molecular sieving (View interaction) annexin B22 and annexin B22 bind by x-ray crystallography (View interaction).
Crystal structure and immunological properties of the first annexin from Schistosoma mansoni: insights into the structural integrity of the schistosomal tegument.,Leow CY, Willis C, Osman A, Mason L, Simon A, Smith BJ, Gasser RB, Jones MK, Hofmann A FEBS J. 2013 Dec 28. doi: 10.1111/febs.12700. PMID:24428567[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Leow CY, Willis C, Osman A, Mason L, Simon A, Smith BJ, Gasser RB, Jones MK, Hofmann A. Crystal structure and immunological properties of the first annexin from Schistosoma mansoni: insights into the structural integrity of the schistosomal tegument. FEBS J. 2013 Dec 28. doi: 10.1111/febs.12700. PMID:24428567 doi:http://dx.doi.org/10.1111/febs.12700
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